{"id":36,"date":"2018-05-30T09:22:39","date_gmt":"2018-05-30T13:22:39","guid":{"rendered":"https:\/\/research.cbc.osu.edu\/hummon.1\/?page_id=36"},"modified":"2026-04-08T12:18:42","modified_gmt":"2026-04-08T16:18:42","slug":"publications","status":"publish","type":"page","link":"https:\/\/research.cbc.osu.edu\/hummon.1\/publications\/","title":{"rendered":"Publications"},"content":{"rendered":"\t\t
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(Updated as of April 8th, 2026)<\/em><\/h6>

2026<\/h2>

95) Maabadi, J. L.; Lopez, A.; Holbrook, J. H.; Hummon, A. B. “Minimizing the Coffee Ring Effect: Improving MALDI Dried-Drop Analysis by Sequential Spotting<\/a>.” Journal of the American Society for Mass Spectrometry. 2026. doi:10.1021\/jasms.6c00063<\/p>

94) Lopez, A.; Hummon, A. B. “MALDI Spatial Proteomics: A Mini Review of Approaches and Techniques<\/a>.” Analytical Methods. 2026. doi:10.1039\/D5AY01341K<\/span><\/p>

2025<\/h2>

93) Platzer, N. E.; Hummon, A. B. “Discovery and targeted Mass spectrometry-based proteomics of Ovarian cancer<\/a>.” Expert Review of Proteomics. 2025. doi:10.1080\/14789450.2025.2602752<\/span><\/p>

92) Whitney, C. B.; Beller, N. C.; Fries, B. D.; Lopez, A.; Hummon, A. B. “Longitudinal Proteomic Changes in HCT 116 Colon Cancer Spheroids During Growth<\/a>.” Journal of Proteome Research. 2025. doi:10.1021\/acs.jproteome.5c00207<\/p>

91) Lopez, A.; Holbrook, J. H.; Hummon, A. B. “MALDI Imaging and Spatial SILAC Proteomics of Three-Dimensional Multicellular Spheroids Dynamically Dosed with Doxorubicin-Encapsulating Liposomes<\/a>.” Analytical Chemistry. 2025. doi:10.1021\/acs.analchem.5c01309<\/p>

90) Fang, F.; Fries, B. D.; Wang, Z.; Liu, X.; Hummon, A. B.; Sun, L. “Quantitative Top-Down Proteomics Reveals Significant Differences in Histone Proteoforms Between Metastatic and Nonmetastatic Colorectal Cancer Cells<\/a>.” Proteomics. 2025. doi:10.1002\/pmic.202400336<\/p>

89) Fries, B. D.; Sekera, E. R.; Holbrook, J.H.; Hummon, A. B. “Multi-Omic Evaluation of PLK1 Inhibitor-Onvansertib-In Colorectal Cancer Spheroids<\/a>.” Journal of Mass Spectrometry. 2025. doi:10.1002\/jms.5137<\/p>

88) Fredriksen, T. R.; Holbrook, J. H.; Rosas, L.; Angeles-Lopez, Q. D.; Mora, A. L.; Rojas, M.; Hummon, A. B. “Light-Reactive Norharmane Derivatization of Lipid Isomers by Matrix-Assisted Laser Desorption\/Ionization Mass Spectrometry<\/a>.” Analytical Chemistry. 2025. doi:10.1021\/acs.analchem.4c05629<\/p>

87) Fries, B. D.; Hummon, A. B. “Mass Spectrometry-Based Applications of Spheroids in Cancer Biology<\/a><\/span>.” Annual Review of Analytical Chemistry<\/span>. 2025.\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 <\/span>doi:10.1146\/annurev-anchem-061424-090547<\/span><\/p>

2024<\/h2>

86) Sekera, E. R.; Rosas, L.; Holbrook, J. H.; Angeles-Lopez, Q. D.; Khaliullin, T.; Rojas, M.; Mora, A. L.; Hummon, A. B. “<\/span>Single Cell MALDI-MSI Analysis of Lipids and Proteins within a Replicative Senescence Fibroblast Model<\/a>.” Journal of the American Society for Mass Spectrometry. 2024. <\/span>doi\/10.1021\/jasms.4c00095.<\/p>

85) Mukherjee, S.; Kulkarni, A.; Holbrook, J. H.; Dasanayake, G. S.; Hu, D.; Tanner, E. E. L.; Hummon, A. B.; Watkins, D. L. “<\/span>Uptake of Self-Assembling Amphiphilic Tryptophan-Based Polymer Nanoparticles in Three-Dimensional Cell Culture Models<\/a>.” ACS Applied Nano Materials. 2024. <\/span>doi\/10.1021\/acsanm.4c04589.<\/p>

84) Lopez, A.; Holbrook, J. H.; Kemper, G. E.; Lukowski, J. K.; Andrews, W. T.; Hummon, A. B. “Tracking Drugs and Lipids: Quantitative Mass Spectrometry Imaging of Liposomal Doxorubicin Delivery and Bilayer Fate in Three-Dimensional Tumor Models.<\/a>” Analytical Chemistry. 2024. doi: 10.1021\/acs.analchem.4c01586.<\/p>

83)Sekera, E. R.; Akkaya-Colak, K.; Lopez, A.; Mihaylova, M.; Hummon, A. B. “Mass Spectrometry Imaging and Histology for the Analysis of Budding Intestinal Organoids<\/a>.” Analytical Chemistry. 2024. doi:\u00a010.1021\/acs.analchem.3c05725.<\/p>

82) Shannon, A.; Boos, C.; Searle, B.; Hummon, A. B. “Gas-phase Fractionation Data-Independent Acquisition analysis of 3D Cocultured Spheroid Tumor Model Reveals Altered Translational Processes and Signaling using Proteomics<\/a>.” Journal of Proteome Research. 2024. doi: 10.1021\/acs.jproteome.3c00786.<\/p>

81) Holbrook, J. H.; Kemper, G.; Hummon, A. B. “Quantitative Mass Spectrometry Imaging: Therapeutics & Biomolecules<\/a>.” Chemical Communications. 2024. doi: 10.1039\/D3CC05988J.<\/p>

2023<\/h2>

80) Fries, B. D.; Tobias, F.; Wang, Y.; Holbrook, J. H.; Hummon, A. B. “Lipodomics Profiling Reveals Differential Alterations after FAS Inhibition in Colon Cancer Cell Culture Models<\/a>.” Journal of Proteome Research. 2023. doi: 10.1021\/10.1021\/acs.jproteome.3c00593.<\/p>

79) Chua, A. E.; Pfeifer, L. D.; Sekera, E. R.; Hummon, A. B.; Desaire H. “Workflow for Evaluating Normalization Tools for Omics Data Using Supervised and Unsupervised Machine Learning.<\/a>” Journal of the American Society for Mass Spectrometry. 2023. doi: 10.1021\/10.1021\/jasms.3c00295.<\/p>

78) Beller, N. C.; Wang, Y.; Hummon, A. B. “Evaluating the Pharmacokinetics and Pharmacodynamics of Chemotherapeutics within a Spatial SILAC-Labeled Spheroid Model System<\/a>.” Anal. Chem. 2023. doi: 10.1021\/acs.analchem.3c00905.<\/p>

77) Holbrook, J. H.; Sekera, E. R.; Lopez, A.; Fries, B. D.; Tobias, F.; Akkaya, K.; Mihaylova, M. M.; Hummon, A. B. “Enhancement of Lipid Signals in Matrix-Assisted Laser Desorption\/Ionization Mass Spectrometry with Ammonium Fluoride a Matrix Additive<\/a>.” Anal. Chem. 2023. doi: 10.1021\/acs.analchem.3c00753.<\/p>

76) Li, Y., et al. “Targeting a xenobiotic transporter to ameliorate vincristine-induced sensory neuropathy<\/a>.” JCI Insight. 2023. doi: 10.1172\/jci.insight.164646.<\/p>

75) Badjic, J. D.; Gunawardana, V. W. L.; Ward, C.; Wang, H.; Holbrook, J. H.; Sekera, E. R.; Cui, H.; Hummon, A. B. “Crystalline Nanoparticles of Water-Soluble Covalent Basket Cages (CBCs) for Encapsulation of Anticancer Drugs<\/a>” Angewandte Chemie International Edition. 2023. doi: 10.1002\/anie.202306722.<\/span><\/p>

74) Wang, Y.; Hummon, A. B. \u201cQuantification of Irinotecan in Single Spheroids Using Internal Standards by MALDI Mass Spectrometry Imaging<\/a>\u201d Analytical Chemistry. 2023. doi: 10.1021\/acs.analchem.3c00699.<\/p>

73) Shortt, R. L.; Wang, Y.; Hummon, A. B.; Jones, L. M. \u201cDevelopment of Spheroid-FPOP: An In-Cell Protein Footprinting Method for 3D Tumor Spheroids<\/a>\u201d Journal of the American Society for Mass Spectrometry. 2023. doi: 10.1021\/jasms.2c00307.<\/p>

72) Zhou, Q.; Alvarez, M.R.S.; Solakyildirim, K.; Tena, J.; Serrano, L.M.N.; Lam, M.;\u00a0 Nguyen, C.; Tobias, F.; Hummon, A.B.; Nacario, R.C.; Lebrilla, C.B. \u201cMulti-Glycomic analysis of spheroid glycocalyx differentiates 2- and 3-dimensional cell models<\/a>\u201d Glycobiology. 2022. doi: 10.1093\/glycob\/cwac075.<\/p>

2022<\/h2>

71) Hempfling, Jordan; Sekera, Emily; Sarkar, Amar; Hummon, Amanda; Pei, Dehua. \u201cGeneration of Proteins with Free N-Terminal Cysteine by Aminopeptidases<\/a>\u201d Journal of the American Chemical Society. 2022.\u00a0doi: 10.1021\/jacs.2c10194.
<\/a><\/p>

70) McCool, E. N.; Xu, T.; Chen, W.; Beller, N. C.; Nolan, S. M.; Hummon, A. B.; Liu, X.; Sun, L. \u201cDeep top-down proteomics revealed significant proteoform-level differences between metastatic and nonmetastatic colorectal cancer cells<\/a>\u201d Science Advances. 2022. doi: 10.1093\/glycob\/cwac075.<\/p>

69) Tobias, F.; Hummon, A.B. \u201cLipidomic Comparison of 2D and 3D Colon Cancer Cell Culture Models<\/a>\u201d Journal of Mass Spectrometry. 2022. 57(8): :e4880. doi: 10.1002\/jms.4880. PMID: 36028991.<\/p>

68) Beller, N.C.; Hummon, A.B. \u201cAdvances in stable isotope labeling: dynamic labeling for spatial and temporal proteomic analysis<\/a>\u201d Mol Omics. 2022. doi: 10.1039\/d2mo00077f.<\/p>

2021<\/h2>

67) Beller, NC; Lukowski, JK; Ludwig, KR; Hummon, AB “Spatial SILAC Isotopic Labeling by Amino Acids in Cell Culture: Pulse Chase Labeling of Three-Dimensional Multicellular Spheroids for Global Proteome Analysis<\/a>” Analytical Chemistry. 2021 Dec 7;\u00a093(48):15990-15999.\u00a0 doi: 10.1021\/acs.analchem.1c03461.<\/span><\/p>

66) Wang, Y; Hummon, AB “MS imaging of multicellular tumor spheroids and organoids as an emerging tool for personalized medicine and drug discovery<\/a>” J Biol Chem. 2021 Oct; 297(4):\u00a0101139.\u00a0 doi: 10.1016\/j.jbc.2021.101139.<\/p>

65).\u00a0Andrews, WT; Bickner, AN; Tobias, F; Ryan, KA; Bruening, ML; Hummon, AB \u201cElectroblotting through enzymatic membranes to enhance molecular tissue imaging<\/a>\u201d<\/span> J Am Soc Mass Spectrom. 2021 Jul 7;32(7):1689-1699. doi: 10.1021\/jasms.1c00046. Epub 2021 Jun 10.<\/span><\/p>

64). Shannon, AE; Boos, CE; Hummon AB “Co-culturing multicellular tumor models: Modeling the tumor microenvironment and analysis techniques<\/a>” Proteomics. 2021 Feb 10:e2000103. doi: 10.1002\/pmic.202000103. Epub ahead of print. PMID: 33569922.<\/p>

63). Bibyk, MJ; Campbell, MJ; Hummon AB “Mass spectrometric investigations of caloric restriction mimetics<\/a>” Proteomics. 2021 Jan 18:e2000121. doi: 10.1002\/pmic.202000121. PMID: 33460282.<\/p>

2020<\/h2>

62). Lindhorst, PH; Hummon, AB “Proteomics of Colorectal Cancer: Tumors, Organoids, and Cell Cultures-A Minireview<\/a>” Front Mol Biosci 2020 Dec 10.\u00a0 PMID: 33363210<\/span><\/p>

61). Dougherty, PG; Wellmerling, JH; Koley, A; Lukowski, JK; Hummon, AB; Cormet-Boyaka, E; Pei, D “<\/span>Cyclic Peptidyl Inhibitors against CAL\/CFTR Interaction for Treatment of Cystic Fibrosis<\/a>” J Med Chem 2020 Dec 24;63(24):15773-15784. PMID: 33314931<\/span><\/p>

60). <\/span>Dougherty, PG, Karpurapu, M; Koley, A; Lukowski, JK; Qian, Z; Nirujogi, TS; Rusu, L; Chung, S; Hummon, AB; Li, HW; Christman, JW; Pei, D “<\/span>A Peptidyl Inhibitor that Blocks Calcineurin-NFAT Interaction and Prevents”\u00a0<\/a>Acute Lung Injury J Med Chem 2020 Nov 12;63(21):12853-12872. PMID: 33073986<\/span><\/p>

59). Bickner, AN; Champion, MM; Hummon, AB; Bruening, ML “Electroblotting through a tryptic membrane for LC-MS\/MS analysis of proteins separated in electrophoretic gels<\/a>” Analyst 2020 Nov 23;145(23):7724-7735. PMID: 33000802<\/p>

58). Tobias, F and Hummon AB “Considerations for MALDI-based Quantitative Mass Spectrometry Imaging Studies<\/a>” J Proteome Res. 2020 19 (9), 3620-3630.\u00a0<\/em>PMID: 32786684<\/span><\/p>

57). Hua, D; Liu, X; Go, EP; Wang, Y; Hummon, AB; Desaire, H “<\/span>How to Apply Supervised Machine Learning Tools to MS Imaging Files: Case Study with Cancer Spheroids Undergoing Treatment with the Monoclonal Antibody Cetuximab<\/a>” J Am Soc Mass Spectrom 2020 Jul 1;31(7):1350-1357. PMID: 32469221<\/span><\/p>

56). Buechler, SA; Stephens, MT; Hummon, AB; Ludwig, K; Cannon, E; Carter, TTC; Resnick, J; G\u00f6kmen-Polar, Y; and Badve, SS.<\/span> “ColoType: a forty gene signature for consensus molecular subtyping of colorectal cancer tumors using whole-genome assay or targeted RNA-sequencing”. <\/a>Sci Rep<\/i> (10), 12123 2020. PMID: 32694712<\/span><\/p>

55). Andrews, WT; Donahue, D; Holmes, A; Balsara, R; Castellino, FJ “In situ metabolite and lipid analysis of GluN2D-\/- and wild-type mice after ischemic stroke using MALDI MSI<\/a>” Anal Bioanal Chem. 2020 Feb 28. PMID: 32107573<\/span><\/p>

2019<\/h2>

54). Tobias, F, McIntosh, JC, LaBonia, GJ, Boyce, MW, Lockett, MR, and Hummon, AB \u201c<\/span>Developing a Drug Screening Platform: MALDI-Mass Spectrometry Imaging of Paper-Based Cultures<\/a>\u201d Anal Chem. 2019, 91(24): 15370\u201315376. PMID: 31755703<\/span><\/p>

53). Lukowski, JK and Hummon AB “Quantitative evaluation of liposomal doxorubicin and its metabolites in spheroids<\/a>.” Anal Bioanal Chem. 2019 411(27): 7087-7094. PMID: 31471684<\/p>

52). Wangsa, D; Braun, R; Stuelten, CH; Brown, M; Bauer, KM; Emons, G; Weston, LA; Hu, Y; Yang, HH; Lee, MP; Bauer, P; Warner, L; Upender, M; Hummon, AB; Camps, J; Ried, T \u201cInduced Chromosomal Aneuploidy Results in Global and Consistent Deregulation of the Transcriptome of Cancer Cells<\/a>\u201d Neoplasia. 2019 21(7): 721-729. PMID: 31174021<\/p>

51). Chen, D; Ludwig, KR; Yang, Z. Shen, X; Hummon, AB; Sun, L \u201cCapillary zone electrophoresis-tandem mass spectrometry for large-scale phosphoproteomics with the production of over 11000 phosphopeptides from the colon carcinoma HCT116 cell line<\/a>\u201d Anal Chem. 2019 91(3): 2201\u20132208.\u00a0PMID:\u00a030624053<\/p>

2018<\/h2>

50). Liu, X; Lukowski, JK; Flinders, C; Mumenthaler, S; Hummon, AB \u201cMALDI-MSI of Immunotherapy: Mapping the EGFR-Targeting Antibody Cetuximab in 3D Colon Cancer Cell Cultures<\/a>\u201d Anal Chem. 2018 90(24): 14156-64. PMID: 30479121<\/p>

49). Shields, PG; Song, MA; Freudenheim, JL; Brasky, TM; Mathe, E; McElroy, JP; Hummon, AB; Wewers, MD \u201cElectronic Cigarettes and the Lung Proteome.<\/a>\u201d Am J Respir Crit Care Med. 2018 198(10):1350-1. PMID: 30153045<\/p>

48).\u00a0 Schroll MM and Hummon AB “<\/span>Employing proteomics to understand the effects of nutritional intervention in cancer treatment<\/a>.” Anal Bioanal Chem\u00a0<\/span>2018 Oct;410(25):6371-6386. PMID: 29974151<\/span><\/p>

47).\u00a0 Schroll MM, Ludwig KR, LaBonia GJ, Herring EL, Hummon AB. “Combined Short-Term Glucose Starvation and Chemotherapy in 3D Colorectal Cancer Cell Culture Decreases 14-3-3 Family Protein Expression and Phenotypic Response to Therapy<\/a>.”J Am Soc Mass Spectrom 2018 29(10): 2012-2022. PMID: 30019162<\/p>

46).\u00a0 Ludwig KR, Schroll MM, Hummon AB.\u00a0“Comparison of In-Solution, FASP, and S-Trap Based Digestion Methods for Bottom-Up Proteomic Studies.”\u00a0<\/a>J Proteome Res<\/span>.2018 Jul 6;17(7):2480-2490<\/span><\/p>

45).\u00a0Schroll MM, Ludwig KR, Bauer KM, Hummon AB. “Calcitriol Supplementation Causes Decreases in Tumorigenic Proteins and Different Proteomic and Metabolomic Signatures in Right versus Left-Sided Colon Cancer.<\/a>” Metabolites. 2018 Jan 11;8(1):5. PMID: 29324674<\/p>

44).\u00a0Feist PE, Loughran EA, Stack MS, Hummon AB. “Quantitative proteomic analysis of murine white adipose tissue for peritoneal cancer metastasis<\/a>.” Anal Bioanal Chem. 2018 Feb;410(5):1583-1594. PMID: 29282499<\/p>

43).\u00a0LaBonia GJ, Ludwig KR, Mousseau CB, Hummon AB. “iTRAQ Quantitative Proteomic Profiling and MALDI-MSI of Colon Cancer Spheroids Treated with Combination Chemotherapies in a 3D Printed Fluidic Device<\/a>.” Anal Chem. 2018 Jan 16;90(2):1423-1430. PMID: 29227110<\/p>

42).\u00a0Liu X, Flinders C, Mumenthaler SM, Hummon AB. “MALDI Mass Spectrometry Imaging for Evaluation of Therapeutics in Colorectal Tumor Organoids.<\/a>” J Am Soc Mass Spectrom. 2018 Mar;29(3):516-526. PMID: 29209911<\/p>

2017<\/h2>

41).\u00a0Andrews WT, Skube SB, Hummon AB. “Magnetic bead-based peptide extraction methodology for tissue imaging.<\/a>” Analyst. 2017 Dec 18;143(1):133-140<\/p>

40).\u00a0Bailey KA, Klymenko Y, Feist PE, Hummon AB, Stack MS, Schultz ZD. “Chemical Analysis of Morphological Changes in Lysophosphatidic Acid-Treated Ovarian Cancer Cells<\/a>.” Sci Rep. 2017 Nov 10;7(1):15295<\/p>

39).\u00a0 Zhang G, Vilim FS, Liu DD, Romanova EV, Yu K, Yuan WD, Xiao H, Hummon AB, Chen TT, Alexeeva V, Yin SY, Chen SA, Cropper EC, Sweedler JV, Weiss KR, Jing J. “Discovery of leucokinin-like neuropeptides that modulate a specific parameter of feeding motor programs in the molluscan model, Aplysia<\/a>.” J Biol Chem. 2017 Nov 17;292(46):18775-18789<\/p>

38). Lukowski JK, Weaver EM, Hummon AB. \u201cAnalyzing Liposomal Drug Delivery Systems in Three-Dimensional Cell Culture Models Using MALDI Imaging Mass Spectrometry<\/a>\u201d. Analytical Chemistry. 2017. In Press.<\/p>

37). Boyce MW, LaBonia GJ, Hummon AB, Lockett MR. \u201cAssessing chemotherapeutic effectiveness using a paper-based tumor model<\/a>\u201d. Analyst. 2017. In Press.<\/p>

36). Schroll MM, LaBonia GJ, Ludwig KR, Hummon AB. \u201cGlucose restriction combined with autophagy inhibition and chemotherapy in HCT 116 spheroids decreases cell clonogenicity and viability regulated by tumor supressor genes<\/a>\u201d. JPR. 2017. In Press.<\/p>

35). Feist P, Sidoli S, Liu X, Schroll M, Rahmy S, Fujiwara R, Garcia B, Hummon AB. \u201cMulticellular Tumor Spheroids Combined with Mass Spectrometric Histone Analysis to Evaluate Epigenetic Drugs<\/a>\u201d . Analytical Chemistry. 2017. Mar 7;89(5):2773-2781.<\/p>

34). Ludwig KR, Hummon AB. \u201cMass Spectrometry for the Discovery of Biomarkers for Sepsis<\/a>\u201d. Molecular Biosystems. 2017. Mar 28;13(4):648-664.<\/p>

2016<\/h2>

33). US Patent #9,464,328. \u201cBiomarkers and Uses Thereof in Prognosis and Treatment Strategies for Right-Side Colon Cancer Disease and Left-Side Colon Cancer Disease\u201d (Co-Inventor Steven Buechler).<\/p>

32). Schunter A, Yue XS, Hummon AB. \u201cPhosphoproteomics of Colon Cancer Metastasis: Comparative Mass Spectrometric Analysis of the Isogenic Primary and Metastatic Cell Lines SW480 and SW620<\/a>\u201d. Analytical and Bioanalytical Chemistry. 2016. Mar;409(7):1749-1763.<\/p>

31). Liu X, Hummon AB.”Chemical Imaging of Platinum-Based Drugs and their Metabolites<\/a>“. Scientific Reports. 2016. Dec 5;6:38507.<\/p>

30). Yue XS, Lukowski JK, Weaver EM, Skube SB, Hummon AB. \u201dQuantitative Proteomic and Phosphoproteomic Comparison of 2D and 3D Colon Cancer Cell Culture Models<\/a>\u201d. Journal of Proteome Research. 2016. Dec 2;15(12):4265-4276..<\/p>

29). Schroll M, Liu X, Herzog S, Skube SB, Hummon AB. \u201cNutrient Restriction of Glucose or Serum Results in Similar Proteomic Expression Changes in 3D Colon Cancer Cell Cultures<\/a>\u201d Nutrition Research. 2016. Oct;36(10):1068-1080.<\/p>

28) . Amanda B Hummon. \u201cToward Integrated Omics\u201d. The Analytical Scientist, Issue 0616. 2016.<\/p>

27). LaBonia GJ, Lockwood SY, Heller AA, Spence DM, Hummon AB. \u201cDrug penetration and metabolism in 3-dimensional cell cultures treated in a 3D printed fluidic device: Assessment of irinotecan via MALDI imaging mass spectrometry<\/a>\u201d. Proteomics. 2016. Jun;16(11-12):1814-21.<\/p>

26). Ludwig KR, Dahl R, Hummon AB. \u201cEvaluation of the mirn23a Cluster through an iTRAQ-based Quantitative Proteomic Approach<\/a>\u201d. Journal of Proteome Research. 2016. May 6; 15(5):1497-505.<\/p>

2015<\/h2>

25). Ludwig KR, Sun L, Zhu G, Dovichi NJ, Hummon AB. \u201cOver 2,300 Phosphorylated Peptide Identifications with Single-Shot Capillary Zone Electrophoresis-Tandem Mass Spectrometry in a 100 min Separation<\/a>\u201d. Analytical Chemistry. 2015. Sept 23; 87(19): 9532-9537.<\/p>

24). Yue XS, Schunter A, Hummon AB. \u201cComparing Multi-Step IMAC and Multi-Step TiO2 Methods for Phosphopeptide Enrichment<\/a>\u201d. Analytical Chemistry. 2015. August 3;87(17):8837-8844.<\/p>

23). Liu X, Hummon AB. \u201cMass Spectrometry Imaging of Therapeutics from Animal Models to Three-Dimensional Cell Cultures\u201d. Analytical Chemistry. 2015. Jun 18;87(19):9508-9519.<\/p>

22). Weaver EM, Hummon AB, Keithley RB. \u201cChemometric Analysis of MALDI Mass Spectrometric Images of Three-Dimensional Cell Culture Systems<\/a>\u201d. Analytical Methods. 2015. Sept 7;7(17):7208-7219.<\/p>

21). Feist P, and Hummon AB \u201cProteomic Challenges: Sample Preparation Techniques for Microgram-Quantity Protein Analysis from Biological Samples<\/a>\u201d. International Journal of Molecular Sciences. 2015. Feb 5;16(2):3537-63.<\/p>

20). Feist P, Sun L, Liu X, Dovichi NJ, Hummon AB. “Bottom-up proteomic analysis of single HCT 116 colon carcinoma multicellular spheroids<\/a>“. Rapid Communications in Mass Spectrometry. 2015. Apr 15;29(7):654-658.<\/p>

2014<\/h2>

19). Liu X, Hummon AB. “Quantitative Determination of Irinotecan and the Metabolite SN-38 by nLC-ESI-MS\/MS in Different Regions of Multicellular Tumor Spheroids<\/a>“. Journal of The American Society for Spectrometry. 2014. Apr; 26(4):577-86.<\/p>

18). Bauer KM, Watts T, Buechler S, Hummon AB. “Proteomic and functional investigation of the colon cancer relapse-associated genes NOX4 and ITGA3<\/a>“. Journal of Proteome Research. 2014 Nov 7; 13 (11);4910-8.<\/p>

17). Ahlf DR, Masyuko RN, Hummon AB, Bohn PW. \u201cCorrelated Mass Spectrometry Imaging and Confocal Raman Microscopy for Studies of Three-Dimensional Cell Culture Sections<\/a>\u201d. Analyst. 2014 Sep 21;139 (18);4578-85.<\/p>

16). Wheatcraft Ahlf DR, Liu X, Hummon AB. \u201cSample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models<\/a>\u201d. Journal of Visualized Experiments. 2014 Dec 5;(94).<\/p>

2013<\/h2>

15). Weston LA, Bauer KM, Skube SB, Hummon AB. \u201cSelective, bead-based global peptide capture using a bifunctional crosslinker<\/a>\u201d. Anal Chem. 2013 Nov 19;85(22):10675-9.<\/p>

14). Weston LA, Hummon AB. “Comparative LC-MS\/MS analysis of Optimal Cutting Temperature (OCT) compound removal for the study of mammalian proteomes<\/a>“. Analyst. 2013 Nov 7;138(21):6380-4.<\/p>

13). Keithley R.B., Weaver E.M., Metzinger M.P., Rosado A.M., Hummon A.B., Dovichi N.J. \u201cSingle Cell Metabolic Profiling of Tumor Mimics.<\/a>\u201d Anal Chem. 2013 Oct 1;85(19);8910-8.<\/p>

12). Yue XS, Hummon A.B. “Combination of Multi-Step IMAC Enrichment with High-pH Reverse Phase Separation for In-Depth Phosphoproteomic Profiling<\/a>“. Journal of Proteome Research. 2013 Sep 6;12(9):4176-86.<\/p>

11). Weston LA*, Bauer KM*, Hummon A.B. “Comparison of bottom-up proteomic approaches for LC-MS analysis of complex proteomes<\/a>“. Analytical Methods, 2013, 5 (18), 4615 – 4621.<\/p>

10). Liu, X, Weaver EM, Hummon A.B.”Evaluation of Therapeutics in Three-Dimensional Cell Culture Systems by MALDI Imaging Mass Spectrometry<\/a>“. Analytical Chemistry, 2013 Jul 2;85(13):6295-302. Epub 2013 Jun 11.<\/p>

9). Hummon, A.B.; Dovichi, N.J. \u201cThe Tools of Proteomics\u201d The Analytical Scientist, 2013, 1, 413-420.<\/p>

8). Dovichi NJ, Hummon A.B. “The Tools Behind Genomics” The Analytical Scientist, 2013, 1, 24-31.<\/p>

7). Weaver EM, Hummon A.B. “Imaging mass spectrometry: from tissue sections to cell cultures<\/a>” Advanced Drug Discovery Reviews, 2013 Jul;65(8):1039-55.<\/p>

2012<\/h2>

6). Yue XS, Hummon A.B. “Mass spectrometry-based phosphoproteomics in cancer research.” Front. Biol. 2012, 7(6): 566\u2013586.<\/p>

5). Bauer KM, Hummon A.B. \u201cEffects of the miR-143\/-145 MicroRNA Cluster on the Colon Cancer Proteome and Transcriptome<\/a>.\u201d J Proteome Res. 2012 Sep 7;11(9):4744-54.<\/p>

4). Bauer KM, Lambert PA, and Hummon A.B. “Comparative label-free LC-MS\/MS analysis of colorectal adenocarcinoma and metastatic cells treated with 5-fluorouracil<\/a>” Proteomics 2012 Jun;12(12):1928-37.<\/p>

3). Hummon AB, Pitt JJ, Camps J, Emons G, Skube SB, Huppi K, Jones TL, Beissbarth T, Kramer F, Grade M, Difilippantonio MJ, Ried T and Caplen NJ. “Systems-wide RNAi analysis of CASP8AP2\/FLASH shows transcriptional deregulation of the replication-dependent histone genes and extensive effects on the transcriptome of colorectal cancer cells<\/a>.” Molecular Cancer 2012 Jan 4;11:1.<\/p>

2). Bauer, K.M.; Hummon, A.B.; Buechler, S. \u201cRight-side and left-side colon cancer follow different pathways to relapse<\/a>.\u201d Mol Carcinog., 2012 May; 51(5);411-21.<\/p>

2011<\/h2>

1). Li H, Hummon A.B. \u201cImaging mass spectrometry of three-dimensional cell culture systems<\/a>.\u201d Anal Chem. 2011 Nov 15;83(22) 8794-801.<\/p>

Post Doc and Graduate Training<\/h2>

18). Padilla-Nash HM, McNeil NE, Yi M, Nguyen QT, Hu Y, Wangsa D, Mack DL, Hummon AB, Case C, Cardin E, Stephens R, Difilippantonio MJ, Ried T. Aneuploidy, Oncogene Amplification, and Epithelial to Mesenchymal Transition Define Spontaneous Transformation of Murine Epithelial Cells.”Carcinogenesis. 2013 Mar 15;73(6);2013-13.<\/p>

17). Camps J, Pitt JJ, Emons G, Hummon AB, Case CM, Grade M, Jones TL, Nguyen QT, Ghadimi BM, Beissbarth T, Difilippantonio MJ, Caplen NJ, Ried T. \u201dGenetic Amplification of the NOTCH Modulator LNX2 Upregulates the WNT\/\u03b2-Catenin Pathway in Colorectal Cancer.\u201d Cancer Res. 2013 Mar 15;73(6):2003-13.<\/p>

16). Grade, M.; Hummon, A.B.; Emons, G.; Camps, J.; Spitzner, M.; Hoermann, P.; Gaedcke, J.; Ebner, R.; Becker, H.; Ghadimi, B.M.; Beissbarth, T.; Difilippantonio, M.J.; Caplen, N.J.; Ried, T. \u201cIdentification and Validation of NOL5A and RPS2 as Potential Therapeutic Targets in Colorectal Cancer using a Functional Genomics Approach.\u201d Int J. Cancer. March 2011 128(5):1069-79.<\/p>

15). Camps, J.; Nguyen, Q.T.; Padilla-Nash, H.M.; Knutsen, T.; McNeil, N.E.; Wangsa, D.; Hummon, A.B.; Grade, M.; Ried, T.; Difilippantonio, M.J. \u201cIntegrative genomics reveals mechanisms of copy number alterations responsible for transcriptional deregulation in colorectal cancer.\u201d Genes Chromosomes Cancer. 2009 Nov;48(11);1002-17.<\/p>

14). Camps, J.; Grade, M.; Nguyen, Q.T.; H\u00f6rmann, P.; Becker, S.; Hummon, A.B.; Rodriguez, V.; Chandrasekharappa, S.; Chen, Y.; Difilippantonio, M.J.; Becker, H.; Ghadimi, B.M.; Ried, T. \u201cChromosome breakpoints in primary colon cancer cluster at sites of structural variants in the genome.\u201d Cancer Res. 2008 Mar 1;68(5):1284-95.<\/p>

13). Hummon, A.B.; Martin, S.E.; Caplen, N.J. \u201cRNAi screens: Going right to the target.\u201d Screening-Trends in Drug Discovery 4, 2 (2007).<\/p>

12). Hummon, A.B.; Lim, S.R.; Difilippantonio, M.J.; Ried, T. \u201cIsolation and solubilization of proteins after TRIzol extraction of RNA and DNA from patient material following prolonged storage.\u201d Biotechniques 42, 467 (2007).<\/p>

11). Grade, M.; H\u00f6rmann, P.; Becker, S.; Hummon, A.B.; Wangsa, D.; Varma, S.; Simon, R.; Liersch, T.; Becker, H.; Difilippantonio, M.J.; Ghadimi, B.M.; Ried, T. \u201cGene Expression Profiling Reveals a Massive, Aneuploidy-Dependent Transcriptional Deregulation and Distinct Differences between Lymph Node Negative and Positive Colon Carcinomas.\u201d Cancer Res. 67, 41 (2007).<\/p>

10). Hummon, A.B.; Richmond, T.A.; Amare, A.; Ewing, M.A.; Verleyen, P.; Baggerman, G.; Schoofs, L.; Southey, B.; Rodriguez-Zas, S.L.; Robinson, G.E.; Sweedler, J.V. \u201cFrom the Genome to the Proteome: Uncovering Peptides in the Apis Brain.\u201d Science 314, 647 (2006).<\/p>

9). The Honeybee Genome Sequencing Consortium. “Insights into social insects from the genome of the honeybee Apis mellifera.” Nature 443, 931 (2006).<\/p>

8). Amare, A.; Hummon, A.B.; Southey, B.R.; Zimmerman, T.A.; Rodriguez-Zas, S.L.; Sweedler, J.V. \u201cBridging Neuropeptidomics and Genomics with Bioinformatics: Prediction of Mammalian Neuropeptide Prohormone Processing.\u201d J. Proteome Res. 5, 1162 (2006).<\/p>

7). Hummon, A.B.; Amare, A.; Sweedler, J.V. \u201cDiscovering New Invertebrate Neuropeptides using Mass Spectrometry.\u201d Mass Spectrom. Rev. 25, 77 (2006).<\/p>

6). Stuart, J.N.; Hummon, A.B.; Sweedler, J.V. \u201cThe Chemistry of Thought: Neurotransmitters in the Brain.\u201d Anal. Chem. 76, 121A (2004).<\/p>

5). Cummins, S.F.; Nichols, A.; Amare, A.; Hummon, A.B.; Sweedler, J.V.; Nagle, G.T. \u201cNovel Candidate Protein Pheromones in Aplysia: Identification, Characterization, Expression, and Biological Activity.\u201d J. Biol. Chem. 279, 25614 (2004).<\/p>

4). Page, J.S.; Hummon, A.B.; Sweedler, J.V. \u201cSingle Cell Peptide Analysis with MALDI-MS\u201d for Encyclopedia of Mass Spectrometry. Vol 2, Biological Applications. Eds. R. Caprioli and M. Gross. 2004.<\/p>

3). Hummon, A.B.; Hummon, N.P.; Corbin, R.W.; Li, L.; Vilim, F.S.; Weiss, K.R.; Sweedler, J.V. \u201cFrom Precursor to Final Peptides: A Statistical Sequence-Based Approach to Predicting Prohormone Processing.\u201d J. Proteome Res. 2, 650 (2003).<\/p>

2). Hummon, A.B.; Corbin, R.W.; Sweedler, J.V. \u201cDiscovering New Neuropeptides using Single Cell Mass Spectrometry.\u201d Trends Anal. Chem. 22, 515 (2003).<\/p>

1). Hummon, A.B.; Huang, H.; Kelley, W.P.; Sweedler. J.V. \u201cA Novel Prohormone Processing Site in Aplysia californica: the Leu-Leu Rule.\u201d J. Neurochem. 82, 1398 (2002).<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

(Updated as of April 8th, 2026) 2026 95) Maabadi, J. L.; Lopez, A.; Holbrook, J. H.; Hummon, A. B. “Minimizing[…]<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-36","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/research.cbc.osu.edu\/hummon.1\/wp-json\/wp\/v2\/pages\/36","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/research.cbc.osu.edu\/hummon.1\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/research.cbc.osu.edu\/hummon.1\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/research.cbc.osu.edu\/hummon.1\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/research.cbc.osu.edu\/hummon.1\/wp-json\/wp\/v2\/comments?post=36"}],"version-history":[{"count":183,"href":"https:\/\/research.cbc.osu.edu\/hummon.1\/wp-json\/wp\/v2\/pages\/36\/revisions"}],"predecessor-version":[{"id":1219,"href":"https:\/\/research.cbc.osu.edu\/hummon.1\/wp-json\/wp\/v2\/pages\/36\/revisions\/1219"}],"wp:attachment":[{"href":"https:\/\/research.cbc.osu.edu\/hummon.1\/wp-json\/wp\/v2\/media?parent=36"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}