{"id":46,"date":"2013-08-01T12:13:15","date_gmt":"2013-08-01T16:13:15","guid":{"rendered":"http:\/\/research.chemistry.ohio-state.edu\/kudryashov\/?page_id=46"},"modified":"2015-04-24T16:36:06","modified_gmt":"2015-04-24T20:36:06","slug":"protocols","status":"publish","type":"page","link":"https:\/\/research.cbc.osu.edu\/kudryashov.1\/protocols\/","title":{"rendered":"Protocols"},"content":{"rendered":"<p><span style=\"color: #888888\"><em><a href=\"https:\/\/research.cbc.osu.edu\/kudryashov.1\/wp-content\/uploads\/2013\/08\/Protocols.jpg\"><img decoding=\"async\" class=\"alignleft  wp-image-1195\" src=\"https:\/\/research.cbc.osu.edu\/kudryashov.1\/wp-content\/uploads\/2013\/08\/Protocols.jpg\" alt=\"Protocols\" width=\"104\" height=\"148\" \/><\/a><\/em><\/span><\/p>\n<p><em>&#8220;You can&#8217;t try to do things; you simply must do them.&#8221; \u00a0\u00a0<span style=\"text-align: right\">Ray Bradbury<\/span><\/em><\/p>\n<p style=\"text-align: right\"><span style=\"color: #888888\"><em>&#8220;The way a man does one thing is the way he does everything.&#8221;<\/em><\/span><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong><a href=\"https:\/\/research.cbc.osu.edu\/kudryashov.1\/protocols\/actin-protocols\/\"><span style=\"color: #000080\">ACTIN PROTOCOLS<\/span><\/a><\/strong><\/p>\n<p><span style=\"color: #000080\"><strong><a href=\"https:\/\/research.cbc.osu.edu\/kudryashov.1\/protocols\/pcr-cloning-mutagenesis\/\"><span style=\"color: #000080\">PCR, CLONING AND\u00a0MUTAGENESIS<\/span><\/a><\/strong><\/span><\/p>\n<p><span style=\"color: #000080\"><strong><a href=\"https:\/\/research.cbc.osu.edu\/kudryashov.1\/protocols\/protein-expression-and-purification\/\"><span style=\"color: #000080\">PROTEIN EXPRESSION AND PURIFICATION<\/span><\/a><\/strong><\/span><\/p>\n<p><span style=\"color: #000080\"><strong><a href=\"https:\/\/research.cbc.osu.edu\/kudryashov.1\/protocols\/sds-page-and-western-blotting\/\"><span style=\"color: #000080\">SDS-PAGE AND WESTERN BLOTTING<\/span><\/a><\/strong><\/span><\/p>\n<p><span style=\"color: #000080\"><strong><a title=\"Mosquito Walkthrough\" href=\"https:\/\/research.cbc.osu.edu\/kudryashov.1\/wp-content\/uploads\/2013\/08\/Mosquito-Walkthrough.docx\"><span style=\"color: #000080\">CRYSTALLOGRAPHY<\/span><\/a><\/strong><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>&#8220;You can&#8217;t try to do things; you simply must do them.&#8221; \u00a0\u00a0Ray Bradbury &#8220;The way a man does one thing is the way he does everything.&#8221; &nbsp; &nbsp; ACTIN PROTOCOLS PCR, CLONING AND\u00a0MUTAGENESIS PROTEIN EXPRESSION AND PURIFICATION SDS-PAGE AND WESTERN BLOTTING CRYSTALLOGRAPHY<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":4,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-46","page","type-page","status-publish","hentry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false},"uagb_author_info":{"display_name":"Dmitri Kudryashov","author_link":"https:\/\/research.cbc.osu.edu\/kudryashov.1\/author\/kudryashov-1osu-edu\/"},"uagb_comment_info":0,"uagb_excerpt":"&#8220;You can&#8217;t try to do things; you simply must do them.&#8221; \u00a0\u00a0Ray Bradbury &#8220;The way a man does one thing is the way he does everything.&#8221; &nbsp; &nbsp; ACTIN PROTOCOLS PCR, CLONING AND\u00a0MUTAGENESIS PROTEIN EXPRESSION AND PURIFICATION SDS-PAGE AND WESTERN BLOTTING CRYSTALLOGRAPHY","_links":{"self":[{"href":"https:\/\/research.cbc.osu.edu\/kudryashov.1\/wp-json\/wp\/v2\/pages\/46","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/research.cbc.osu.edu\/kudryashov.1\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/research.cbc.osu.edu\/kudryashov.1\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/research.cbc.osu.edu\/kudryashov.1\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/research.cbc.osu.edu\/kudryashov.1\/wp-json\/wp\/v2\/comments?post=46"}],"version-history":[{"count":1,"href":"https:\/\/research.cbc.osu.edu\/kudryashov.1\/wp-json\/wp\/v2\/pages\/46\/revisions"}],"predecessor-version":[{"id":1435,"href":"https:\/\/research.cbc.osu.edu\/kudryashov.1\/wp-json\/wp\/v2\/pages\/46\/revisions\/1435"}],"wp:attachment":[{"href":"https:\/\/research.cbc.osu.edu\/kudryashov.1\/wp-json\/wp\/v2\/media?parent=46"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}