{"id":2260,"date":"2023-05-18T15:22:30","date_gmt":"2023-05-18T19:22:30","guid":{"rendered":"https:\/\/research.cbc.osu.edu\/wysocki.11\/?page_id=2260"},"modified":"2023-05-18T15:23:55","modified_gmt":"2023-05-18T19:23:55","slug":"instrument-modification","status":"publish","type":"page","link":"https:\/\/research.cbc.osu.edu\/wysocki.11\/instrument-modification\/","title":{"rendered":"Instrument Modification"},"content":{"rendered":"\n

SID-IM\u00a0<\/strong><\/p>\n\n\n\n

Ion mobility (IM) is a gas-phase separation technique that separates ions based on their charge, size, and shape. In our lab, we couple IM to surface-induced dissociation (SID)1\u20134<\/sup> to increase understanding about protein complex structure and subunit connectivity. IM can separate the overlapped protein complex precursor and subunit products, which improves post-SID data interpretation and protein complex characterization (Figure 1.<\/strong>). Additionally, with IM, we are able to perform surface-induced unfolding (SIU) to study the protein complex disorder by applying a range of SID voltages, recording the shift of the arrival time or collision cross section (CCS), and generating the SIU fingerprint (Figure 2.<\/strong>). <\/p>\n\n\n\n

\"\"<\/a><\/figure>\n\n\n\n

Figure <\/em>1<\/em>. Top) SID spectrum of the 11-mer holoTRAP; Bottom) SID Mobiligram of the 11-mer holoTRAP<\/em> <\/p>\n\n\n\n

\"\"<\/a><\/figure>\n\n\n\n

Figure <\/em>2<\/em>. SIU fingerprint of 11+ homotetramer streptavidin<\/em> <\/p>\n\n\n\n

References <\/p>\n\n\n\n

(1) Snyder, D. T.; Jones, B. J.; Lin, Y.-F.; Cooper-Shepherd, D. A.; Hewitt, D.; Wildgoose, J.; Brown, J. M.; Langridge, J. I.; Wysocki, V. H. Surface-Induced Dissociation of Protein Complexes on a Cyclic Ion Mobility Spectrometer. Analyst<\/em> 2021<\/strong>, 146<\/em> (22), 6861\u20136873. https:\/\/doi.org\/10.1039\/D1AN01407B. <\/p>\n\n\n\n

(2) Zhou, M.; Huang, C.; Wysocki, V. H. Surface-Induced Dissociation of Ion Mobility-Separated Noncovalent Complexes in a Quadrupole\/Time-of-Flight Mass Spectrometer. Anal. Chem.<\/em> 2012<\/strong>, 84<\/em> (14), 6016\u20136023. https:\/\/doi.org\/10.1021\/ac300810u. <\/p>\n\n\n\n

(3) Zhou, M.; Jones, C. M.; Wysocki, V. H. Dissecting the Large Noncovalent Protein Complex GroEL with Surface-Induced Dissociation and Ion Mobility\u2013Mass Spectrometry. Anal. Chem.<\/em> 2013<\/strong>, 85<\/em> (17), 8262\u20138267. https:\/\/doi.org\/10.1021\/ac401497c. <\/p>\n\n\n\n

(4) Zhou, M.; Wysocki, V. H. Surface Induced Dissociation: Dissecting Noncovalent Protein Complexes in the Gas Phase. Acc. Chem. Res.<\/em> 2014<\/strong>, 47<\/em> (4), 1010\u20131018. https:\/\/doi.org\/10.1021\/ar400223t. <\/p>\n","protected":false},"excerpt":{"rendered":"

SID-IM\u00a0 Ion mobility (IM) is a gas-phase separation technique that separates ions based on their charge, size, and shape. In our lab, we couple IM to surface-induced dissociation (SID)1\u20134 to increase understanding about protein complex structure and subunit connectivity. IM can separate the overlapped protein complex precursor and subunit products, which improves post-SID data interpretation … Continue reading “Instrument Modification”<\/span><\/a><\/p>\n","protected":false},"author":32,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2260","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-json\/wp\/v2\/pages\/2260","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-json\/wp\/v2\/users\/32"}],"replies":[{"embeddable":true,"href":"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-json\/wp\/v2\/comments?post=2260"}],"version-history":[{"count":3,"href":"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-json\/wp\/v2\/pages\/2260\/revisions"}],"predecessor-version":[{"id":2267,"href":"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-json\/wp\/v2\/pages\/2260\/revisions\/2267"}],"wp:attachment":[{"href":"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-json\/wp\/v2\/media?parent=2260"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}