{"id":431,"date":"2012-11-15T03:34:01","date_gmt":"2012-11-15T03:34:01","guid":{"rendered":"http:\/\/research.chemistry.ohio-state.edu\/wysocki\/?page_id=431"},"modified":"2015-07-03T18:11:44","modified_gmt":"2015-07-03T22:11:44","slug":"sid","status":"publish","type":"page","link":"https:\/\/research.cbc.osu.edu\/wysocki.11\/group-home\/projects\/sid\/","title":{"rendered":"SID and Protein Structure"},"content":{"rendered":"<p><img decoding=\"async\" src=\"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-content\/uploads\/2015\/07\/crpcartoonTOC_TNH.png\" alt=\"Surface Induced Dissociation of Large Proteins\" \/><\/p>\n<p>&nbsp;<\/p>\n<p>Subunit organization of protein complexes can be studied with tandem mass spectrometry by disruption of the quaternary structure in a controlled manner. The major challenge of using this method for structural analysis of non-covalent protein complexes is to overcome the undesired unfolding of subunits that occurs in the commonly used collision activation with gasous targets (Collision Induced Dissociation, CID), which results in loss of information on the original conformation. Our custom surface induced dissociation (SID) device has shown promising results of revealing sub-architecutre of protein complexes with minimal unfolding. The ultimate goal of this research is to develop a better mass spectrometric tool for\u00a0improved structural characterization of large protein complexes.<\/p>\n<p><strong>Ongoing Projects in the group<\/strong><\/p>\n<ul>\n<li><a title=\"Click to view more details\" href=\"https:\/\/research.cbc.osu.edu\/wysocki.11\/group-home\/projects\/sid\/sid_im\/\">Surface induced dissociation coupled with ion mobility<\/a><\/li>\n<li><a title=\"Click to see a list of projects\" href=\"https:\/\/research.cbc.osu.edu\/wysocki.11\/group-home\/projects\/sid\/proteincomplex\/\">Protein-protein and Protein-ligand complexes<\/a><\/li>\n<li><a title=\"Download PDF description\" href=\"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-content\/uploads\/2012\/09\/Surface-induced-dissociation-of-multimeric-protein.pdf\" target=\"_blank\">Surface-induced dissociation of multimeric protein complexes<\/a>\u00a0(PDF)<\/li>\n<li><a title=\"Download PDF description\" href=\"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-content\/uploads\/2012\/09\/Chemical-Crosslinking-Probing-the-interface-of-proteins.pdf\" target=\"_blank\">Chemical Crosslinking \u2013 Probing the interface of Proteins<\/a>\u00a0(PDF)<\/li>\n<\/ul>\n<p><strong>Previous Projects<\/strong><\/p>\n<p>Please click to download the PDF for detailed descriptions<\/p>\n<ul>\n<li><a title=\"Download PDF description\" href=\"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-content\/uploads\/2012\/09\/Surface-Induced-dissociation-in-a-quadrupole-time-of-flight-mass-spectrometer.pdf\" target=\"_blank\">Surface-Induced dissociation in a quadrupole time-of-flight mass spectrometer<\/a><\/li>\n<li><a title=\"Download PDF description\" href=\"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-content\/uploads\/2012\/09\/Protein-interactions-probed-by-mass-spectrometric-techniques-hydrogen-deuterium-exchange-limited-proteolysis-and-crosslinking.pdf\" target=\"_blank\">Protein interactions probed by mass spectrometric techniques: hydrogen deuterium exchange, limited proteolysis and crosslinking<\/a><\/li>\n<li><a title=\"Download PDF description\" href=\"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-content\/uploads\/2012\/09\/Self-assembled-monolayers-SAMs-as-collision-surfaces-for-ion-activation.pdf\" target=\"_blank\">Self-assembled monolayers (SAMs) as collision surfaces for ion activation<\/a><\/li>\n<li><a title=\"Download PDF description\" href=\"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-content\/uploads\/2012\/09\/Characterizing-electron-transfer-properties-of-molecular-wires-through-ion-surface-collisions.pdf\" target=\"_blank\">Characterizing electron transfer properties of molecular wires through ion-surface collisions<\/a><\/li>\n<li><a title=\"Download PDF description\" href=\"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-content\/uploads\/2012\/09\/Surface-induced-dissociation-SID-using-derivatized-silicon-substrates.pdf\" target=\"_blank\">Surface-induced dissociation (SID) using derivatized silicon substrates<\/a><\/li>\n<li><a title=\"Download PDF description\" href=\"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-content\/uploads\/2012\/09\/Surface-induced-dissociation-kinetics.pdf\" target=\"_blank\">Surface-induced dissociation kinetics<\/a><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; Subunit organization of protein complexes can be studied with tandem mass spectrometry by disruption of the quaternary structure in a controlled manner. The major challenge of using this method for structural analysis of non-covalent protein complexes is to overcome the undesired unfolding of subunits that occurs in the commonly used collision activation with gasous &hellip; <a href=\"https:\/\/research.cbc.osu.edu\/wysocki.11\/group-home\/projects\/sid\/\" class=\"more-link\">Continue reading<span class=\"screen-reader-text\"> &#8220;SID and Protein Structure&#8221;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":386,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-431","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-json\/wp\/v2\/pages\/431","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-json\/wp\/v2\/comments?post=431"}],"version-history":[{"count":8,"href":"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-json\/wp\/v2\/pages\/431\/revisions"}],"predecessor-version":[{"id":952,"href":"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-json\/wp\/v2\/pages\/431\/revisions\/952"}],"up":[{"embeddable":true,"href":"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-json\/wp\/v2\/pages\/386"}],"wp:attachment":[{"href":"https:\/\/research.cbc.osu.edu\/wysocki.11\/wp-json\/wp\/v2\/media?parent=431"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}