Welcome to the Badu Lab!
The Badu Research Group is focused on the development of new mass spectrometry (MS) tools for use by MS non-experts. We welcome undergraduate and graduate students, and postdoctoral fellows who are interested in this field, and may want to be part of this effort to contact the PI. See recent news.
Recent innovation in mass spectrometry (MS) is the ability to generate intact molecular ions, focus and use them as ordinary reagents for organic reactions at ambient surface, outside the mass spectrometer. Most projects in the Badu lab build on this innovation, but instead of simple organic compounds, we focus on drugs and biomolecules of specific biological importance.
For example, selected proteins and antibodies can be aerosolized, and the resulting molecular cations/anions are directed onto a surface (paper, glass, tissue sections, etc.) for specific molecular recognition. Such experiments are expected to allow rapid and sensitive detection of drugs, tumor markers and antigens of infectious diseases. We are designing novel molecular probes for ambient MS analysis and imaging of proteins, antibodies, antigens, nucleic acids, and other disease biomarkers. The probes are rationally designed to facilitate (1) point-of-care and direct-to-customer applications with handheld mass spectrometers, and (2) a novel on-demand disease diagnostic concept that permits onsite friendly sample collection followed by analysis at a later time without affecting diagnostic outcome. Major efforts in our lab are also dedicated to the introduction of charged aerosols for treating respiratory diseases. This research has potential to advance our current understanding in aerosol therapy, and may yield new focus for general drug synthesis in which molecular ions, instead of their neutral counterparts, are used in medicine.
Additional projects in the Badu lab include: (1) droplet chemistry and accelerated chemical synthesis – a new contained-electrospray apparatus is developed that allow ion generation and reaction in a single step. This new process of sample ionization enables removal of ion suppression effects in electrospray ionization, yielding ion intensities that reflect analyte concentration in solution; (2) fundamental ion chemistry at atmospheric pressure – a new picomole scale, real time photoreaction screening platform is developed that enable rapid photoreaction monitoring using mass spectrometry; and (3) development of new ambient ionization techniques – several novel ionization methods have been developed with special emphasis on direct analysis of miniscule volumes of biological samples from ambient surfaces. Current applications include therapeutic drug monitoring, and pharmacokinetic analysis of chemotherapeutic drugs (e.g., paclitaxel and lenalidomide).
These three experiments form the backbone of the Badu lab and all students become equipped with modern MS techniques, including the newly emerging field of gas-phase ionic chemistry outside the mass spectrometer, and its application in immunoassay, bio-conjugation, peptide/protein cross-linking, etc.
Selected Publications (click here for complete publication list):
1. “Reactive charged droplets for reduction of matrix effects in electrospray ionization mass spectrometry“ Dmytro S. Kulyk, Colbert F. Miller, and Abraham K. Badu-Tawiah* Anal. Chem. 2015, 87(21), 10988–10994
2. He, Q.; Badu-Tawiah, AK*.; Chen, S.; Xiong, C.; Liu, H.; Zhou, Y.; Hou, J.; Zhang, N.; Li, Y.; Xie, X.; Wang, J.; Mao, L.; Nie, Z.* In-situ Bio-conjugation and Ambient Surface Modification using Reactive Charged Droplets Anal. Chem. 2015, accepted
3. Chen, S.; Xiong, C.; Liu, H.; Wan, Q.; Hou, J.; Wang, J.; He, Q.; Badu-Tawiah, A.; Nie, Z.* Mass Spectrometry Imaging Reveals the Sub-organ Distribution of Carbon Nanomaterials Nature Nanotechnology, 2015, 10, 176-182
4. Badu-Tawiah,# K.; Lathwal,# S.; Kaastrup, K.; Al-Sayah, M.; Christodouleas, D.C.; Smith, B.S.; Whitesides, M.G.*; Sikes, D.H.* Polymerization-based signal amplification for paper-based immunoassays Lab Chip, 2015, 15, 655-659. #co-first authors
5. Jjunju, F.P.M.; Maher, S.; Li, A.; Badu-Tawiah, K.; Taylor*, S.; Cooks, R.G.* Analysis of Polycyclic Aromatic Hydrocarbons Using Desorption Atmospheric Pressure Chemical Ionization Coupled to a Portable Mass Spectrometer J. Am. Soc. Mass Spectrom. 2015, 26, 271-280
6. AK Badu-Tawiah, LS Eberlin, Z Ouyang, RG Cooks Chemical aspects of the extractive methods of ambient ionization mass spectrometry Annu. Rev. Phys. Chem. 2013, 64, 481-505.
7. AK Badu-Tawiah, A Li, FPM Jjjunju, RG Cooks Peptide Cross‐Linking at Ambient Surfaces by Reactions of Nanosprayed Molecular Cations Angew. Chem. 2012,124 (37), 9551-9555.
8. T Müller, AK Badu‐Tawiah, RG Cooks Accelerated Carbon-Carbon Bond‐Forming Reactions in Preparative Electrospray Angew. Chem. Int. Ed. 2012, 51(47), 11832-11835.
9. AK Badu-Tawiah, DI Campbell, RG Cooks Reactions of microsolvated organic compounds at ambient surfaces: Droplet velocity, charge state, and solvent effects J. Am. Soc. Mass Spect. 2012, 23(6), 1077-1084
On the Cover:
- Prof. Zongxiu Nie (Institute of Chemistry, Chinese Academy of Science, Beijing, China)
- Dr. Simon Maher (Electrical Engineering and Electronics, University of Liverpool, UK)
- Prof. Stephen Taylor (Q-Technologies, UK)
- Prof. Martin Thuo (Materials Science and Engineering, Iowa State University)
- Prof. Mitch Phelps (College of Pharmacy, The Ohio State University)
- Prof. Mark Drew (Microbial Infection and Immunity, The Ohio State University)