DISC_gDNA_Generator

• Takes an RNA sequence as input to generate a list of DNA guides for DISC mediated cleavage covering the length of the RNA.
• Download gDNAgen_v1.1.exe
  • You will need to have Python2.x installed.
  • Double-click on the *.exe file.
  • Select a text file with your sequence.
    • The file will need to have only the sequence on the first line and nothing else in the document.
  • At the next prompt, type in the name of your desired output file without an extension (e.g. “output” will yield the file output.txt).

RiboCAT

• RiboCAT  –  (Ribonucleic acid Capillary-electrophoresis Analysis Tool): A new, user-friendly tool for analysis of RNA probing data collected using capillary electrophoresis.
• RiboDOG  –  (RiboCAT Data Output Generator): A companion tool for RiboCAT that formats outputs for error-checking and input files for RNAStructure.
• Please reference the following:
  • Cantara, W. A., Hatterschide, J., Wu, W. & Musier-Forsyth, K. RiboCAT: a new capillary electrophoresis data analysis tool for nucleic acid probing. RNA 23, 240-249, doi:10.1261/rna.058404.116 (2017).

• Downloads
  • RiboCAT RNA Probing Tools
    • Download RiboCAT_v1.42 (updated Oct 18, 2016)
      • Due to new security rules, an additional installation step is required for Excel for Mac 2016 users (see User Manual for details) – Download script HERE.
    • Download RiboDOG_v1.20 (updated Dec 15, 2016)
      • Some features not compatible with Excel for Mac 2016 (see User Manual for details)
    • Download User_Manual/Quick_Start_Guide/Step-by-Step_Guide
    • Download Tutorial_Files (requires unzipping software)
    • • This works with Windows and Mac.
      • RiboCAT requires Python 2.X, and it must be in your PATH.
      • Report bugs to willc.tools@gmail.com
    • View the video tutorial below (updated Oct 18, 2016; now with ability to navigate to different “chapters” of the tutorial):