Nuclear Magnetic Resonance (NMR)

Available instruments

• Bruker Ascend 850 MHz – CBEC 090
  • Triple resonance inverse TCI cryoprobe
• Bruker Ascend 800 MHz – CBEC 090
  • Solid-state probe
• Bruker DRX 800 MHz – Riffe 137
  • Triple resonance inverse TXI cryoprobe
• Bruker DRX 600 MHz – Riffe 137
  • Triple resonance observe TXO cryoprobe
• Bruker Ascend 700 MHz – Riffe 137
  • Triple resonance inverse TXI cryoprobe

Additional Information

• Software: TopSpin 3.2
• More information at CCIC-NMR

Scheduling

• Online calendar and scheduling at fom.osu.edu

Isothermal Titration Calorimetry (ITC)

General ITC Information

Essential reading for any ITC user

• Planning your ITC experiment
• Sample Preparation Tips (Vanderbilt)
• ITC Sample Prep Calculator

Useful Articles

• ITC review (Jelesarov & Bosshard, 1999)
  • Excellent introductory review of calorimetry
• ITC review (Holdgate (Microcal), 2001)
  • -Background, analysis, and interpretation
• Microcal ITC analysis using Origin software, 2004
  • -Detailed analysis using Origin software
• Microcal ITC troubleshooting guide (Microcal)
  • -For commonly encountered problems

Microcal VP-ITC

Information

• Biochemistry department usage
• 1.4mL cell volume, 285μL syringe volume
• Microcal VP-ITC User Manual

Scheduling

• Contact Antonia Duran / 732 Riffe / 688-8146
• Check usage calendar.
• VP ITC Training Quiz

Microcal ITC200

Information

• Foster and Henkin Lab usage
• 280μL cell volume, 60μL syringe volume
• Microcal iTC200 User Manual
• Investigation of periodicity in iTC200 injections
• Figures of high noise at temperatures >50°C
• Comparison with TA instrument Nano ITC

Scheduling

• Contact Antonia Duran / 732 Riffe / 688-8146
• Check usage calendar.
• ITC200 Training Quiz

Core Facilities

• Biophysical Interaction and Characterization Facility (BICF)
• Campus Chemical Instrument Center (CCIC)

SAXS

Ian Kleckner’s SAXS tutorial files: saxs_tutorial.tar