Current Members


Venkat Gopalan

Venkat Gopalan

Principal Investigator
Email
Education and Training


Visiting Scientist – Medical Research Council (MRC) Laboratory of Molecular Biology (Cambridge, UK)


•   Advisor: Sir Aaron Klug
•   Conducted crystallographic studies of bacterial RNase P


Postdoctoral Research Associate – Yale University (New Haven, CT)


•   Advisor: Dr. Sidney Altman
•   Mapped RNA-protein interactions in Escherichia coli RNase P


Ph.D. – University of New Mexico (Albuquerque, NM)


•   Program: Biochemistry
•   Advisor: Dr. Robert H. Glew
•   Dissertation: Mechanism of action of mammalian cytosolic ß-glucosidase: implications for biotransformation of toxic plant glucosides


B.S. – University of Madras (Madras, India)


•   Major: Chemistry


Publications

•   Publications since starting his own independent research group at OSU are listed here.
•   Publications from his time as a graduate student and postdoctoral researcher are listed here.

Lien B. Lai

Lien B. Lai

Senior Research Scientist
Email


•   lai.104@osu.edu
Current Projects


•   Supervisor: Dr. Mark P. Foster
•   Determine how a mutation in a central nervous system (CNS)-specific tRNA-Arg, which has been linked to neurodegeneration in mice, impacts tRNA biogenesis [collaboration with Dr. Susan L. Ackerman (UCSD)]
•   Utilize sequence analyses, hydroxyl radical-mediated footprinting experiments, and native mass spectrometry to identify novel structural elements in halophilic archaeal RNase P RNAs [collaboration with Drs. Charles J. Daniels and Vicki H. Wysocki (OSU)]
Education and Training


Postdoctoral Researcher – The Ohio State University (Columbus, OH)


•   Department: Biochemistry
•   Advisor: Dr. Venkat Gopalan
•   Purified and characterized plant RNase P
•   Assessed the efficacy of external guide sequences (EGS) and M1GS for targeted RNA degradation in plants


Postdoctoral Researcher – The Ohio State University (Columbus, OH)


•   Department: Plant Biology
•   Advisor: Dr. Fred D. Sack
•   Identified and characterized the Arabidopsis thaliana FOUR LIPS gene that restricts the number of divisions by the guard mother cell to one, thereby preventing the appearance of “four lips”, which arise from two (or more) cell divisions


Ph.D. – Yale University (New Haven, CT)


•   Program: Molecular, Cellular, and Developmental Biology (MCDB)
•   Advisor: Dr. Timothy M. Nelson
•   Dissertation: Regulation of cell-specific expression of C4 photosynthetic genes in Flaveria: a comparative study


B.S. – University of New Mexico (Albuquerque, NM)


•   Major: Biochemistry
•   Advisor: Dr. Robert H. Glew
•   Thesis: Continuous spectrophotometric assays for ß-glucosidases acting on the plant glucosides L-picein and prunasin


Publications


•   Daniels CJ*, Lai LB, Chen T-H, and Gopalan V. (2019) Both kinds of RNase P in all domains of life: Surprises galore. RNA, 25: 286-291.
•   Lai LB*, Tanimoto A^, Lai SM^, Chen W-Y, Marathe IA, Westhof E, Wysocki VH, and Gopalan V*. (2017) A novel double kink-turn module in euryarchaeal RNase P RNAs. Nucleic Acids Res., 45: 7432-7440. [SI] ^joint second authors
•   Lei Q, Lee E, Keerthisinghe S, Lai L, Li M, Lucas JR, Wen X, Ren X*, and Sack FD. (2015) The FOUR LIPS and MYB88 transcription factor genes are widely expressed in Arabidopsis thaliana during development. Am. J. Bot., 102: 1521-1528.
•   Manivannan SN, Lai LB, Gopalan V*, and Simcox A*. (2015) Transcriptional control of an essential ribozyme in Drosophila reveals an ancient evolutionary divide in animals. PLoS Genet., 11: e1004893. [SI 1, 2]
•   Lai SM, Lai LB, Foster MP*, and Gopalan V*. (2014) The L7Ae protein binds to two kink-turns in the Pyrococcus furiosus RNase P RNA. Nucleic Acids Res., 42: 13328-13338. [SI]
•   Ma X°, Lai LB°, Lai SM°, Tanimoto A, Foster MP, Wysocki VH*, and Gopalan V*. (2014) Uncovering the stoichiometry of Pyrococcus furiosus RNase P, a multi-subunit catalytic ribonucleoprotein complex, by surface-induced dissociation and ion mobility mass spectrometry. Angew. Chem. Int. Ed. Engl., 53: 11483-11487. [SI] °joint first authors
•   Govinda Rajulu MB°, Lai LB°, Murali TS, Gopalan V*, and Suryanarayanan TS*. (2014) Several fungi from fire-prone forests of southern India can utilize furaldehydes. Mycol. Prog., 13: 1049-1056. °joint first authors
•   Han B°, Ujor V°, Lai LB, Gopalan V, and Ezeji TC*. (2013) Use of proteomic analysis to elucidate the role of calcium in acetone-butanol-ethanol fermentation by Clostridium beijerinckii NCIMB 8052. Appl. Environ. Microbiol., 79: 282-293. [SI] °joint first authors
•   Chen W-Y, Singh D, Lai LB, Stiffler MA, Lai HD, Foster MP, and Gopalan V*. (2012) Fidelity of tRNA 5′-maturation: a possible basis for the functional dependence of archaeal and eukaryal RNase P on multiple protein cofactors. Nucleic Acids Res., 40: 4666-4680. [SI]
•   Lai LB, Bernal-Bayard P, Mohannath G, Lai SM, Gopalan V*, and Vioque A*. (2011) A functional RNase P protein subunit of bacterial origin in some eukaryotes. Mol. Genet. Genomics, 286: 359-369.
•   Lai LB°, Chan PP°, Cozen AE, Bernick DL, Brown JW, Gopalan V*, and Lowe TM*. (2010) Discovery of a minimal form of RNase P in Pyrobaculum. Proc. Natl. Acad. Sci. USA, 107: 22493-22498. [SI] (Highlighted on the cover and in a commentary) °joint first authors
•   Cho I-M, Lai LB, Susanti D, Mukhopadhyay B, and Gopalan V*. (2010) Ribosomal protein L7Ae is a subunit of archaeal RNase P. Proc. Natl. Acad. Sci. USA, 107: 14573-14578. [SI]
•   Lai LB, Cho I-M, Chen W-Y, and Gopalan V*. (2010) Archaeal RNase P: a mosaic of its bacterial and eukaryal relatives. In F Liu and S Altman (Eds.), Ribonuclease P (Protein Reviews, vol. 10; pp. 153-172). New York, NY: Springer Science + Business Media, LLC.
•   McClain WH*, Lai LB, and Gopalan V. (2010) Trials, travails, and triumphs: an account of RNA catalysis in RNase P. J. Mol. Biol., 397: 627-646.
•   Lai LB, Vioque A, Kirsebom LA*, and Gopalan V*. (2010) Unexpected diversity of RNase P, an ancient tRNA processing enzyme: challenges and prospects. FEBS Lett., 584: 287-296. (Invited article for a special issue)
•   Gopichandran V, Lai LB, and Gopalan V*. (2006) Protein-energy malnutrition. In RH Glew and MD Rosenthal (Eds.), Clinical Studies in Medical Biochemistry, (3rd edition; pp. 255-265). New York, NY: Oxford University Press.
•   Lai LB, Gopichandran V, and Gopalan V*. (2006) Tangier disease: a disorder in the reverse cholesterol transport pathway. In RH Glew and MD Rosenthal (Eds.), Clinical Studies in Medical Biochemistry, (3rd edition; pp. 159-166). New York, NY: Oxford University Press.
•   Lai LB, Nadeau JA, Lucas J, Lee E-K, Nakagawa T, Zhao L, Geisler M, and Sack FD*. (2005) The Arabidopsis R2R3 MYB proteins FOUR LIPS and MYB88 restrict divisions late in the stomatal cell lineage. Plant Cell, 17: 2754-2767.
•   Pulukkunat DK, Stephen Raj ML, Pattanayak D, Lai LB, and Gopalan V*. (2003) Exploring the potential of plant RNase P as a functional genomics tool. In E. Grotewold (Ed.), Plant Functional Genomics (Methods in Molecular Biology, vol. 236; pp. 295-309). Totowa, NJ: Humana Press, Inc.
•   Tsai H-Y°, Lai LB°, and Gopalan V*. (2002) A modified pBluescript-based vector for facile cloning and transcription of RNAs. Anal. Biochem., 303: 214-217. °joint first authors
•   Lai LB, Tausta SL, and Nelson TM*. (2002) Differential regulation of transcripts encoding cytosolic NADP-malic enzyme in C3 and C4 Flaveria species. Plant Physiol., 128: 140-149.
•   Lai LB, Wang L, and Nelson TM*. (2002) Distinct but conserved functions for two chloroplastic NADP-malic enzyme isoforms in C3 and C4 Flaveria species. Plant Physiol., 128: 125-139.
•   McGonigle B, Lai LB, and Nelson T. (1996) Sequences of seven cDNAs (Accession Nos. U29933 to U29939) encoding the rubisco small subunit from Flaveria pringlei. Plant Physiol., 111: 1354 (Plant Gene Register PGR96-057).
•   Lai LB, Gopalan V, and Glew RH*. (1992) Continuous spectrophotometric assays for ß-glucosidases acting on the plant glucosides L-picein and prunasin. Anal. Biochem., 200: 365-369.
Edric K. Choi

Edric K. Choi

Research Assistant
Email
Current Projects


•   Develop a fluorescence-based high-throughput RNase P cleavage assay
Education and Training


B.S. – Denison University (Granville, OH)


•   Major: Biochemistry
•   Advisor: Dr. Rachel M. Mitton-Fry
•   Thesis: Analyzing the role of G•U base pairs on the melting behavior of the Salmonella enterica htrA RNA thermometer


B.A. – Denison University (Granville, OH)


•   Major: Computer Science
•   Advisor: Dr. Jessen Havill
Publications

•   Choi EK, Ulanowicz KA, Nguyen YAH, Frandsen JK, and Mitton-Fry RM*. (2017) SHAPE analysis of the htrA RNA thermometer from Salmonella enterica. RNA., 23: 1569-1581.

Stella M. Lai

Stella M. Lai

Research Assistant
Email


•   lai.231@osu.edu
Current Projects


•   Supervisor: Dr. Vicki H. Wysocki
•   Conduct native mass spectrometry, activity assays, and hydroxyl radical-mediated footprinting experiments with various sub-assemblies of archaeal RNase P complexes to characterize the stoichiometry, binding, and biological roles of individual subunits
Education and Training


Research Assistant – The Ohio State University (Columbus, OH)


•   Department: Chemistry and Biochemistry
•   Advisors: Drs. Mark P. Foster and Venkat Gopalan
•   Mapped interaction sites of RNase P proteins on RNase P RNA and precursor tRNAs using hydroxyl radical-mediated footprinting experiments
•   Determined the subunit stoichiometry of archaeal RNase P using electrophoretic mobility shift assays and native mass spectrometry
•   Examined the effect of magnesium on the interactions between RNase P RNA, proteins, and precursor tRNA substrate


M.S. – The Ohio State University (Columbus, OH)


•   Program: Anatomy
•   Advisors: Drs. Laura C. Boucher and Stephen J. Kolb
•   Thesis: Defective tRNA processing by RNase P contributes to neurodegeneration in mice


B.A. – University of Chicago (Chicago, IL)


•   Major: Biological Sciences


Publications

•   Agu CV, Lai SM, Ujor V, Biswas PK, Jones A, Gopalan V, and Ezeji TC*. (2018) Development of a high-throughput assay for rapid screening of butanologenic strains. Sci. Rep., 8: 3379. [SI]
•   Lai LB*, Tanimoto A^, Lai SM^, Chen W-Y, Marathe IA, Westhof E, Wysocki VH, and Gopalan V*. (2017) A novel double kink-turn module in euryarchaeal RNase P RNAs. Nucleic Acids Res., 45: 7432-7440. [SI] ^joint second authors
•   Samanta MP°*, Lai SM°, Daniels CJ, and Gopalan V*. (2016) Sequence analysis and comparative study of the protein subunits of archaeal RNase P. Biomolecules, 6: 22. [SI] °joint first authors
•   Lai SM, Lai LB, Foster MP*, and Gopalan V*. (2014) The L7Ae protein binds to two kink-turns in the Pyrococcus furiosus RNase P RNA. Nucleic Acids Res., 42: 13328-13338. [SI]
•   Ma X°, Lai LB°, Lai SM°, Tanimoto A, Foster MP, Wysocki VH*, and Gopalan V*. (2014) Uncovering the stoichiometry of Pyrococcus furiosus RNase P, a multi-subunit catalytic ribonucleoprotein complex, by surface-induced dissociation and ion mobility mass spectrometry. Angew. Chem. Int. Ed. Engl., 53: 11483-11487. [SI] °joint first authors
•   Lai LB, Bernal-Bayard P, Mohannath G, Lai SM, Gopalan V*, and Vioque A*. (2011) A functional RNase P protein subunit of bacterial origin in some eukaryotes. Mol. Genet. Genomics, 286: 359-369.

Walter J. Zahurancik

Walter J. Zahurancik

Postdoctoral Researcher
Email
Current Projects


•   Investigate the functional contributions of protein subunits to substrate recognition by archaeal RNase P RNA using single-molecule fluorescence-based techniques [collaboration with Dr. Michael G. Poirier (OSU)]
Education and Training


Postdoctoral Researcher – The Ohio State University (Columbus, OH)


•   2019-2020 OSU Center for RNA Biology (CRB) Researcher
•   2020-2022 Pelotonia Postdoctoral Research Fellow


Ph.D. – The Ohio State University (Columbus, OH)


•   Program: Ohio State Biochemistry Program
•   Advisor: Dr. Zucai Suo
•   Dissertation: Kinetic characterization of human DNA polymerase Ε


B.S. – The Ohio State University (Columbus, OH)


•   Major: Biochemistry
•   Advisor: Dr. Zucai Suo
•   Thesis: Characterization of replicative and error-prone DNA polymerases


Undergraduate Research Assistant – The Ohio State University (Columbus, OH)


•   Department: Biochemistry
•   Advisor: Dr. Zhengrong Wu
•   Cloned, overexpressed, and purified mutants of a nucleotide hydrolase (RCL) for biochemical studies aimed at defining its mechanism of action


Publications

•   Kimsey IJ°, Szymanski ES°, Zahurancik WJ, Shakya A, Xue Y, Chu CC, Sathyamoorthy B, Suo Z*, and Al-Hashimi HM*. (2018) Dynamic basis for dG•dT misincorporation via tautomerization and ionization. Nature, 554: 195-201. °joint first authors
•   Campbell BB°, Light N°, Fabrizio D, Zatzman M, Fuligni F, de Borja R, Davidson S, Edwards M, Elvin JA, Hodel KP, Zahurancik WJ, Suo Z, Lipman T, Wimmer K, Kratz CP, Bowers DC, Laetsch TW, Dunn GP, Johanns TM, Grimmer MR, Smirnov IV, Larouche V, Samuel D, Bronsema A, Osborn M, Stearns D, Raman P, Cole KA, Storm PB, Yalon M, Opocher E, Mason G, Thomas GA, Sabel M, George B, Ziegler DS, Lindhort S, Issai VM, Constantini S, Toledano H, Elhasid R, Farah R, Dvir R, Dirks P, Huang A, Galati MA, Chung J, Ramaswamy V, Irwin MS, Aronson M, Durno C, Taylor MD, Rechavi G, Maris JM, Bouffet E, Hawkins C, Costello JF, Meyn MS, Pursell ZF, Malkin D, Tabori U*, and Shlien A*. (2017) Comprehensive analysis of hypermutation in human cancer. Cell, 171: 1042-1056.e10. °joint first authors
•   Wu J, Wang P, Li L, Williams NL, Ji D, Zahurancik WJ, You C, Wang J, Suo Z, and Wang Y. (2017) Replication studies of carboxymethylated DNA lesions in human cells. Nucleic Acids Res., 45: 7276-7284.
•   Vyas R, Reed AJ, Raper AT, Zahurancik WJ, Wallenmeyer PC, and Suo Z*. (2017) Structural basis for the D-stereoselectivity of human DNA polymerase ß. Nucleic Acids Res., 45: 6228-6237.
•   Tokarsky EJ, Gadkari VV, Zahurancik WJ, Malik CK, Basu AK, and Suo Z*. (2016) Pre-steady-state kinetic investigation of bypass of a bulky guanine lesion by human Y-family DNA polymerases. DNA Repair (Amst.), 46: 20-28.
•   Dunn MR, Larsen AC, Zahurancik WJ, Fahmi NE, Meyers M, Suo Z, and Chaput JC*. (2015) DNA polymerase-mediated synthesis of unbiased threose nucleic acid (TNA) polymers requires 7-deazaguanine to suppress G:G mispairing during TNA transcription. J. Am. Chem. Soc., 137: 4014-4017.
•   Zahurancik WJ, Baranovskiy AG, Tahirov TH, and Suo Z*. (2015) Comparison of the kinetic parameters of the truncated catalytic subunit and holoenzyme of human DNA polymerase Ε. DNA Repair (Amst.), 29: 16-22.
•   Zahurancik WJ, Klein SJ, and Suo Z*. (2014) Significant contribution of the 3’→5′ exonuclease activity to the high fidelity of nucleotide incorporation catalyzed by human DNA polymerase Ε. Nucleic Acids Res., 42: 13853-13860.
•   Vyas R°, Zahurancik WJ°, and Suo Z*. (2014) Structural basis for the binding and incorporation of nucleotide analogs with L-stereochemistry by human DNA polymerase Λ. Proc. Natl. Acad. Sci. USA, 111: E3033-3042. °joint first authors
•   Zahurancik WJ, Klein SJ, and Suo Z*. (2013) Kinetic mechanism of DNA polymerization catalyzed by human DNA polymerase Ε. Biochemistry, 52: 7041-7049.
•   Göksenin AY, Zahurancik WJ, LeCompte KG, Taggart DJ, Suo Z, and Pursell ZF*. (2012) Human DNA polymerase Ε is able to efficiently extend from multiple consecutive ribonucleotides. J. Biol. Chem., 287: 42675-42684.
•   Doddapaneni K, Zahurancik W, Haushalter A, Yuan C, Jackman J, and Wu Z. (2011) RCL hydrolyzes 2′-deoxyribonucleoside 5′-monophosphate via formation of a reaction intermediate. Biochemistry, 50: 4712-4719.

Sravya Kovvali

Sravya Kovvali

Graduate Student
Email
Current Projects


•   Elucidate the mechanism of 6-phosphofructose lysine (FrlB) deglycase to gain insight into fructose-lysine metabolism in bacteria
•   Study the mechanisms of kinases that process Amadori compounds
Education and Training


Graduate Student – The Ohio State University (Columbus, OH)


•   Program: Microbiology Graduate Program


B.Tech. – D.Y. Patil University (Mumbai, India)


•   Major: Biotechnology


Student Researcher – Indian Institute of Technology (IIT) Bombay (Mumbai, India)


•   Department: Biosciences and Bioengineering
•   Advisor: Dr. Kiran Kondabagil
•   Purified and characterized a phage N4 endonuclease that is believed to be a component of the phage genome packaging system


Student Researcher – D.Y. Patil University (Mumbai, India)


•   Department: Biotechnology and Bioinformatics
•   Advisors: Mustansir Bhori and Dr. Kanchanlata Singh
•   Genotyped the single nucleotide polymorphisms (SNPs) involved in type 2 diabetes


Jamison Law

Jamison Law

Graduate Student
Email


•   law.199@osu.edu
Current Projects


•   Crystallize Salmonella deglycase FraB to determine its structure, elucidate its enzymatic mechanism, and identify potential inhibitors
Education and Training


Graduate Student – The Ohio State University (Columbus, OH)


•   Program: Ohio State Biochemistry Program (OSBP)


Research Assistant and Lab Manager – The Ohio State University (Columbus, OH)


•   Department: Chemistry and Biochemistry
•   Advisor: Dr. Venkat Gopalan
•   Purified Homo sapiens RNase P protein subunit RPP21 for antibody production and determined via Western blot analysis that the resultant antibodies were RPP21-specific


M.S. – Purdue University Fort Wayne (Fort Wayne, IN)


•   Program: Biological Sciences
•   Advisor: Dr. Jaiyanth Daniel
•   Thesis: The mycobacterial Rv1551 glycerol-3-phosphate acyltransferase enhances phospholipid biosynthesis in cell lysates of Escherichia coli


B.S. – Purdue University Fort Wayne (Fort Wayne, IN)


•   Major: Biological Sciences
•   Advisor: Dr. Jaiyanth Daniel


Publications

•   Law JD and Daniel J*. (2017) The mycobacterial Rv1551 glycerol-3-phosphate acyltransferase enhances phospholipid biosynthesis in cell lysates of Escherichia coli. Microb. Pathog., 113: 269-275.

Ila A. Marathe

Ila A. Marathe

Graduate Student
Email
Current Projects


•   Utilize activity assays, SHAPE-Seq experiments, and ensemble and single-molecule FRET measurements to study the effect of magnesium on inter-domain contacts of Pyrococcus furiosus (Pfu) RNase P RNA and on precursor tRNA cleavage [collaboration with Drs. Julius B. Lucks (Northwestern) and Michael G. Poirier (OSU)]
•   Characterize Methanobrevivacter smithii RNase P, a potential anti-obesity drug target
Education and Training


Graduate Student – The Ohio State University (Columbus, OH)


•   Program: Microbiology Graduate Program
•   2018-2020 Pelotonia Graduate Student Research Fellow


Junior Research Fellow – Agharkar Research Institute (Pune, India)


•   Department: Center for Nanobiosciences
•   Advisor: Dr. Vandana Ghormade
•   Optimized synthesis of chitosan and poly(lactic-co-glycolic acid) (PLGA) nanoparticles for use as carriers of RNA-based insecticides
•   Studied uptake of fluor-labeled nanoparticles by insect cell lines


M.S. – Abasaheb Garware College of Arts and Science (Pune, India)


•   Program: Microbiology
•   Advisors: Dr. S.S. Diwanay and Prof. V.G. Kshirsagar
•   Thesis: Probiotic idli fermentation using Weissella isolate


B.S. – Abasaheb Garware College of Arts and Science (Pune, India)


•   Major: Microbiology


Publications

•   Lai LB*, Tanimoto A^, Lai SM^, Chen W-Y, Marathe IA, Westhof E, Wysocki VH, and Gopalan V*. (2017) A novel double kink-turn module in euryarchaeal RNase P RNAs. Nucleic Acids Res., 45: 7432-7440. [SI] ^joint second authors
•   Kamat V, Marathe I, Ghormade V*, Bodas D*, and Paknikar K*. (2015) Synthesis of monodisperse chitosan nanoparticles and in situ drug loading using active microreactor. ACS Appl. Mater. Interfaces, 7: 22839-22847.

Hong-Duc Phan

Hong-Duc Phan

Graduate Student
Email
Current Projects


•   Identify residues in archaeal RNase P proteins that contact the RNase P RNA and/or the precursor tRNA substrate [collaboration with Dr. Vicki H. Wysocki (OSU)]
•   Study the role of protein cofactors on human RNase P catalysis
Education and Training


Graduate Student – The Ohio State University (Columbus, OH)


•   Program: Ohio State Biochemistry Program
•   2019-2021 Pelotonia Graduate Student Research Fellow


M.S. – Seoul National University (Seoul, South Korea)


•   Program: Pharmacy
•   Advisor: Dr. Sunghyouk Park
•   Thesis: Metabolomic effects of skn-1 down regulation in C. elegans


B.S. – University of Medicine and Pharmacy of Ho Chi Minh City (Ho Chi Minh City, Vietnam)


•   Major: Pharmacy
•   Advisor: Dr. Nguyen Minh Duc
•   Thesis: Experimental design, relationship analysis, and multi-response optimization for the solvent extraction procedure of Danshen (Salvia miltiorrizha, Bunge.)


Publications

•   Phan H-D, Li J, Poi M, and Nakanishi K*. (2018) Quantification of miRNAs co-immunoprecipitated with Argonaute proteins using SYBR Green-based qRT-PCR. In K. Okamura and K. Nakanishi (Eds.), Argonaute Proteins (Methods in Molecular Biology, vol. 1680; pp. 29-40). New York, NY: Humana Press (Springer Science + Business Media, LLC).
•   Park MS, Phan H-D, Busch F, Hinckley SH, Brackbill JA, Wysocki VH, and Nakanishi K*. (2017) Human Argonaute3 has slicer activity. Nucleic Acids Res., 45: 11867-11877.
•   Kwon HN°, Phan H-D°, Xu WJ, Ko Y-J, and Park S*. (2016) Application of a smartphone metabolomics platform to the authentication of Schisandra sinensis. Phytochem. Anal., 27: 199-205. °joint first authors
•   Wen H°, Xu WJ°, Jin X, Oh S, Phan CHD, Song J, Lee SK, and Park S*. (2015) The roles of IP3 receptor in energy metabolic pathways and reactive oxygen species homeostasis revealed by metabolomic and biochemical studies. Biochim. Biophys. Acta, 1853: 2937-2944. °joint first authors

Vaishnavi Sidharthan

Vaishnavi Sidharthan

Graduate Student
Email
Current Projects


•   Reconstitute and characterize RNase P from mesophilic archaea
•   Test novel cyclic polypeptides as ribozyme delivery vehicles for developing a potential anti-cancer drug [collaboration with Dr. Dehua Pei (OSU)]
Education and Training


Graduate Student – The Ohio State University (Columbus, OH)


•   Program: Chemistry and Biochemistry Graduate Program (Biological Division)


B.S. and M.S. – Indian Institute of Science Education and Research (Mohali, India)


•   Program: Chemistry (BS-MS Dual Degree Program)
•   Advisor: Dr. Santanu Kumar Patel
•   Thesis: Detection of As(III) ions using liquid crystal-based sensing system


Blake E. Szkoda

Blake E. Szkoda

Graduate Student
Email
Current Projects


•   Uncover the regulatory mechanism of putative Salmonella transcription factor FraR
Education and Training


Graduate Student – The Ohio State University (Columbus, OH)


•   Program: Ohio State Biochemistry Program (OSBP)
•   2018-2019 OSU Cellular, Biochemical, and Molecular Sciences Training Program (CMBP) Research Fellow


B.S. – Marietta College (Marietta, OH)


•   Major: Biochemistry
•   Minor: Environmental Science
•   Certificate: Leadership Studies
•   Advisor: Dr. Kimberly Suzanne George Parsons
•   Thesis: The effects of citral on caspase-3 activation in M624 and HaCaT cells


Publications

•   Wu J°, Sabag-Daigle A°, Borton MA^, Kop LFM^, Szkoda BE^, Deatherage Kaiser BL, Lindemann SR, Renslow RS, Wei S, Nicora CD, Weitz KK, Kim Y-M, Adkins JN, Metz TO, Boyaka P, Gopalan V, Wrighton KC, Wysocki VH*, and Ahmer BMM*. (2018) Salmonella-mediated inflammation eliminates competitors for fructose-asparagine in the gut. Infect. Immun., 86: e00945-17. °joint first authors; ^joint second authors


 
 

Laboratory Support Staff


Edric K. Choi

Edric K. Choi

Lab Manager
Email
Current Projects


•   Develop a fluorescence-based high-throughput RNase P cleavage assay
Education and Training


B.S. – Denison University (Granville, OH)


•   Major: Biochemistry
•   Advisor: Dr. Rachel M. Mitton-Fry
•   Thesis: Analyzing the role of G•U base pairs on the melting behavior of the Salmonella enterica htrA RNA thermometer


B.A. – Denison University (Granville, OH)


•   Major: Computer Science
•   Advisor: Dr. Jessen Havill
Publications

•   Choi EK, Ulanowicz KA, Nguyen YAH, Frandsen JK, and Mitton-Fry RM*. (2017) SHAPE analysis of the htrA RNA thermometer from Salmonella enterica. RNA., 23: 1569-1581.

Si Yu Tan

Si Yu Tan

Lab Aide
Email


•   tan.586@osu.edu
Education and Training


Undergraduate Student – The Ohio State University (Columbus, OH)


•   Major: Medical Laboratory Science


Undergraduate Research Assistant – The Ohio State University (Columbus, OH)


•   Department: Chemistry and Biochemistry
•   Supervisor: Dr. Richard Lease
•   Assists with experimental design, preparation, and set-up for BIOCHEM 5621 (Biochemistry and Molecular Biology Laboratory)


Undergraduate Research Assistant – The Ohio State University (Columbus, OH)


•   Department: Chemistry and Biochemistry
•   Advisor: Dr. Venkat Gopalan
•   Conducted enzymatic assays to study the ionic liquid tolerance of fungal endophyte-derived xylosidases
Publications

•   Sengupta A, Zabala A^, Tan SY^, Broadstock A^, Suryanarayanan TS, and Gopalan V*. (2017) Characterization of an ionic liquid-tolerant ß-xylosidase from a marine-derived fungal endophyte. Biochem. Cell Biol., 95: 585-591. ^joint second authors


 
 

Summer 2019 Students


Eleanor Bashian
Undergraduate Student
Biochemistry (major)
Rachel Braun
Undergraduate Student
Biochemistry (major)
Daisy DiVita
Rotation Student
OSBP
Davidnhan To
Rotation Student
CBC (Biological Div.)