American Society for Mass Spectrometry annual conference in Indianapolis June 4-8 2017.
Oral presentations from the Wysocki group
MOE pm 3:30; Probing protein structure via surface induced dissociation and gas phase hydrogen-deuterium exchange in a hybrid FT-ICR; Jing Yan*; Arpad Somogyi; Vicki H. Wysocki; The Ohio State University, Columbus, OH
TOH pm 2:50; Implementation of an Ion Carpet Array for Facile and Compact Surface-Induced Dissociation; Joshua D. Gilbert*; Alyssa Q. Stiving; Vicki H. Wysocki; The Ohio State University, Columbus, OH
WOB pm 3:30; Quantification of fructose-asparagine, an acrylamide precursor, in human foods, animal foods and mouse ceca; Jikang Wu*; Anice Sabag-Daigle; Brian Ahmer; Vicki H. Wysocki; The Ohio State University, Columbus, OH
ThOE am 9:50; Investigation of Protein-Protein Interactions using Surface Induced Dissociation – Mass Spectrometry; Stacey Nash [1]; Florian Busch* [1]; Kristina Heyn [2]; Fabian Ruperti [2]; Rainer Merkl [2]; Reinhard Sterner [2]; Vicki Wysocki [1]; [1] The Ohio State University, Columbus, OH; [2] Institute of Biophysics and Physical Biochemistry, University of Regensburg, Regensburg, Germany
ThOC pm 3:10; Characterization of surface induced dissociation (SID) as a top-down fragmentation technique; Alyssa Q. Stiving*; Joshua D. Gilbert; Vicki H. Wysocki; The Ohio State University, Columbus, OH
ThOG pm 4:10; Analysis of Proteins, Protein Complexes and Protein-Ligand Interactions using Size Exclusion Chromatography-High Resolution Mass Spectrometry; Aniruddha Sahasrabuddhe* [1]; Florian Busch [1]; Zachary VanAernum [1]; Brian Rivera [2]; Vicki Wysocki [1]; [1] The Ohio State University, Columbus, OH; [2] Phenomenex, Inc., 411 Madrid Ave.,, Torrance, CA
Poster presentations from the Wysocki group
WP 203 Probing The Charge Distribution on the Gas-Phase Dissociation Products of Protein Dimers; Mengxuan Jia*; Yang Song; Aniruddha Sahasrabuddhe; Zachary VanAernum; Zibo Chen; David Baker; Vicki H. Wysocki; Ohio State University, Columbus, OH
WP 546 Stoichiometry of HIV-1 Gag polyprotein-RNA complexes as determined by native mass spectrometry; Samantha H Hinckley*; Erik D Olson; Karin Musier-Forsyth; Vicki H. Wysocki; The Ohio State University, Columbus, OH
ThP 021 Analysis of Cellular and Exosomal Proteins to Decode LKB1 Regulated Pathways in Lung Cancer Invasion and Metastasis; Bernice Agana* [1]; Michael Koenig [2]; Takehito Shukuya [2]; Joseph Amann [2]; David Carbone [2]; Vicki Wysocki [1]; [1] Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH; [2] Department of Internal Medicine, The Ohio State University, Columbus, OH
ThP 071 Protein Structure Prediction Guided by Covalent Labeling and SID Mass Spectrometry Data: Elucidation of Protein Structure from Sparse Experimental Data; Melanie Aprahamian; Justin Seffernick; Samantha Hinckley; Sophie Harvey; Vicki Wysocki; Steffen Lindert*; The Ohio State University, Columbus OH
ThP 119 Enrichment of Hydrophobic Endogenous Peptides in Urine from Patients with Severe Preeclampsia by Mesoporous Silica Chips; Hongwu Jing* [1]; Irina A. Buhimschi [2]; Guomao Zhao [2]; Megan Locke [2]; Sten Heinze [2]; Jason Sakamoto [3]; Catalin S. Buhimschi [4]; Vicki H. Wysocki [1]; [1] The Ohio State University, Columbus, OH; [2] Center for Perinatal Research, The Research Institute at Nationwide Children’s Hospital, Columbus, OH; [3] NanoMedical Systems, Houston, TX; [4] Department of Obstetrics and Gynecology, The Ohio State University, Columbus, OH
ThP 613 Development of Surface Induced Dissociation on a High Mass Range Orbitrap Platform with Applications Toward the Study of Protein Complexes; Zachary L. VanAernum* [1]; Joshua D. Gilbert [1]; Mikhail E. Belov [2]; Alexander A. Makarov [2]; Stevan R. Horning [2]; Vicki H. Wysocki [1]; [1] The Ohio State University, Columbus, OH; [2] Thermo Fisher Scientific, Germany
ThP 627 Combining native mass spectrometry with mechanistic binding models to define microscopic thermodynamics of homotropic allostery in oligomeric proteins; Melody Holmquist* [1]; Bryant Jacob [1]; Eilhu C. Ihms [1]; Paul Gollnick [2]; Vicki H. Wysocki [1]; Mark P. Foster [1]; [1] The Ohio State University, Columbus, OH; [2] University at Buffalo, Buffalo, NY
ThP 637 Complex conformations: Ion mobility mass spectrometry provides insight into a conformationally dynamic protein complex; Sophie R Harvey*; Varun V Gadkari; Zucai Suo; Vicki H Wysocki; The Ohio State University, Columbus, OH
ThP 680 Comparison of Different Bottom-Up Proteomics Pipelines and LDA-Based Clustering for Optimal Segregation of Preeclampsia Subphenotypes; Sten Heinze*[1]; Hongwu Jing[2]; Wolfgang Rumpf [3]; Will Ray [3]; Catalin S. Buhimschi [4]; Irina A. Buhimschi [1]; Vicki H. Wysocki [2]; [1] Center for Perinatal Research, The Research Institute at Nationwide Children’s Hospital, Columbus, OH; [2] The Ohio State University, Columbus, OH; [3] TheBattelle Center for Mathematical Medicine, The Research Institute at Nationwide Children’s Hospital, Columbus, OH; [4] Department of Obstetrics and Gynecology, The Ohio State University, Columbus, OH