SID and Protein Structure

Surface Induced Dissociation of Large Proteins


Subunit organization of protein complexes can be studied with tandem mass spectrometry by disruption of the quaternary structure in a controlled manner. The major challenge of using this method for structural analysis of non-covalent protein complexes is to overcome the undesired unfolding of subunits that occurs in the commonly used collision activation with gasous targets (Collision Induced Dissociation, CID), which results in loss of information on the original conformation. Our custom surface induced dissociation (SID) device has shown promising results of revealing sub-architecutre of protein complexes with minimal unfolding. The ultimate goal of this research is to develop a better mass spectrometric tool for improved structural characterization of large protein complexes.

Ongoing Projects in the group

Previous Projects

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