Most Recent Publications First
Wang, Q.; Wang, Q.; Qi, Z.; Moeller, W.; Wysocki, V.H.; Sun, L. “Native Proteomics by Capillary Zone Electrophoresis-Mass Spectrometry.” Angewandte Chemie. 2024, Volume136, Issue48. DOI: 10.1002/ange.202408370. [Request reprint © (pdf)]
Shaw, J. B.; Harvey, S. R.; Du, C.; Xu, Z.; Edgington, R. M.; Olmedillas, E.; Saphire, E. O.; Wysocki, V. H. Protein Complex Heterogeneity and Topology Revealed by Electron Capture Charge Reduction and Surface Induced Dissociation. ACS Cent. Sci. 2024, 10 (8), 1537–1547. DOI: 10.1021/acscentsci.4c00461. [Request reprint © (pdf)]
Li, Y.; Shen, Z.; Zhang, M.; Yang, X.-Y.; Cleary, S. P.; Xie, J.; Marathe, I. A.; Kostelic, M.; Greenwald, J.; Rish, A. D.; Wysocki, V. H.; Chen, C.; Chen, Q.; Fu, T.-M.; Yu, Y. “PtuA and PtuB Assemble into an Inflammasome-like Oligomer for Anti-Phage Defense.” Nat Struct Mol Biol 2024, 31 (3), 413–423. DOI: 10.1038/s41594-023-01172-8. [Request reprint © (pdf)]
Roy, J.; Marathe, I.; Wysocki, V.; Pradeep, T. “Observing Atomically Precise Nanocluster Aggregates in Solution by Mass Photometry.” Chem. Commun. 2024, 60 (52), 6655–6658. DOI: 10.1039/D4CC00363B. [Request reprint © (pdf)]
McElroy, C. A.; Ihms, E. C.; Kumar Yadav, D.; Holmquist, M. L.; Wadhwa, V.; Wysocki, V. H.; Gollnick, P.; Foster, M. P. “Solution Structure, Dynamics and Tetrahedral Assembly of Anti-TRAP, a Homo-Trimeric Triskelion-Shaped Regulator of Tryptophan Biosynthesis in Bacillus Subtilis.” Journal of Structural Biology: X 2024, 10, 100103. DOI: 10.1016/j.yjsbx.2024.100103. [Request reprint © (pdf)]
Yang, X.-Y.; Shen, Z.; Xie, J.; Greenwald, J.; Marathe, I.; Lin, Q.; Xie, W. J.; Wysocki, V. H.; Fu, T.-M. “Molecular Basis of Gabija Anti-Phage Supramolecular Assemblies.” Nat Struct Mol Biol 2024. DOI: 10.1038/s41594-024-01283-w. [Request reprint © (pdf)]
Harvey, S. R.; Gadkari, V.V.; Ruotolo, B.T.; Russell, D.H.; Wysocki, V.H.; Zhou, M. “Expanding Native Mass Spectrometry to the Masses.”Journal of the American Society for Mass Spectrometry 2024 35 (3), 646-652. DOI: 10.1021/jasms.3c00352. [Request reprint © (pdf)]
Du, C.; Cleary, S. P.; Kostelic, M.M.; Jones, B.J.; Kafader, J.O.; Wysocki, V.H. “Combining Surface-Induced Dissociation and Charge Detection Mass Spectrometry to Reveal the Native Topology of Heterogeneous Protein Complexes.” Analytical Chemistry 2023 95 (37), 13889-13896. DOI: 10.1021/acs.analchem.3c02185. [Request reprint © (pdf)]
Insights into the catalytic mechanism of a bacterial deglycase essential for utilization of fructose-lysine”. Protein Science 2023, 32( 7):e4695. DOI:10.1002/pro.4695. [Request reprint © (pdf)]
Gopalan, V. “Kreutzer, A. G.; Guaglianone, G.; Yoo, S.; Parrocha, C. M. T.; Ruttenberg, S. M.; Malonis, R. J.; Tong, K.; Lin, Y.-F.; Nguyen, J. T.; Howitz, W. J.; Diab, M. N.; Hamza, I. L.; Lai, J. R.; Wysocki, V. H.; Nowick, J. S. “Probing differences among Aβ oligomers with two triangular trimers derived from Aβ”. PNAS 2023, 120 (22) e2219216120. DOI: 10.1073/pnas.2219216120. [Request reprint © (pdf)]
Jimenez-Harrison, D.; Huseby, C. J.; Hoffman, C. N.; Sher, S.; Snyder, D.; Seal, B.; Yuan, C.; Fu, H.; Wysocki, V.; Giorgini, F.; Kuret, J. “DJ-1 Molecular Chaperone Activity Depresses Tau Aggregation Propensity through Interaction with Monomers”. Biochemistry 2023, 62 (5), 976–988. DOI: 10.1021/acs.biochem.2c00581. [Request reprint © (pdf)]
Bermeo, S.; Favor, A.; Chang, Y.; Norris, A.; Boyken, S.; Hsia, Y.; Haddox, H. K.; Xu, C.; Brunette, T.j.; Wysocki, V.; Bhabha, G.; Ekiert, D.C.; Baker, D.; “De Novo Design of Obligate ABC-Type Heterotrimeric Proteins.” Nature Structural & Molecular Biology 2022, 29(12), 1266–76. DOI: 10.1038/s41594-022-00879-4. [Request reprint © (pdf)]
, E.F.; , A.; , M.;, K.; , A.;, K.C.; , V.H.; [Request reprint © (pdf)] ahmer, B.M.M. “Sugar-Phosphate Toxicities Attenuate Salmonella Fitness in the Gut. “Journal of Bacteriology, 2022, e00344-22,204, 12. DOI: 10.1128/jb.00344-22.
Sarni, S.; Roca, J.; Du, C; Jia, M.; Li, H.; Damjanovic, A.; Matecka, E.M.; Wysocki, V.; Woodson, S.A.; “Intrinsically Disordered Interaction Network in an RNA Chaperone Revealed by Native Mass Spectrometry.” Proceedings of the National Academy of Sciences 2022 119(47). DOI: 10.1073/pnas.2208780119. [Request reprint © (pdf)]
Thirugnanasambantham, P.; Kovvali, S.; Cool, A.; Gao, Y.; Sabag-Daigle, A.; Boulanger, E.F.; Mitton-Fry, M.; Capua, A.D.; Behrman, E.J.; Wysocki, V.H.; Lindert, S.; Ahmer, B.M.M.; Gopalan, V. “Serendipitous Discovery of a Competitive Inhibitor of FraB, a Salmonella Deglycase and Drug Target.” Pathogens 2022, 11(10), 1102. DOI: 10.3390/pathogens11101102. [Request reprint © (pdf)]
Caldwell, B. J.; Norris, A. S.; Karbowski, C. F.; Wiegand, A. M.; Wysocki, V. H.; Bell, C. E. Structure of a Rect/Redβ Family Recombinase in Complex with a Duplex Intermediate of DNA Annealing. Nature Communications 2022, 13 (1). [Request reprint © (pdf)]
Li, W. ; Norris, A.S; Lichententhal K.; Kelly, S. ; Ihms, E. C. ; Gollnick P. ; Wysocki, V.H. ; Foster, M. P. Thermodynamic Coupling between Neighboring Binding Sites in Homo‐Oligomeric Ligand Sensing Proteins from Mass Resolved Ligand‐Dependent Population Distributions. Protein Science 2022 31, (10) https://doi.org/10.1002/pro.4424. [Request reprint © (pdf)]
Marciano, S.; Dey, D.; Listov, D.; Fleishman, S. J.; Sonn-Segev, A.; Mertens, H.; Busch, F.; Kim, Y.; Harvey, S. R.; Wysocki, V. H.; Schreiber, G. Protein Quaternary Structures in Solution Are a Mixture of Multiple Forms. Chemical Science, 2022,13, 11680-11695 DOI:10.1039/D2SC02794A. [Request reprint © (pdf)]
Turzo, S. M. B. A.; Seffernick, J. T.; Rolland, A. D.; Donor, M. T.; Heinze, S.; Prell, J. S.; Wysocki, V. H.; Lindert, S., Protein shape sampled by ion mobility mass spectrometry consistently improves protein structure prediction. Nature Communications 2022, 13 (1), 4377. DOI:10.1038/s41467-022-32075-9. [Request reprint © (pdf)]
Guaglianone, G.; Torrado, B.; Lin, Y.-F.; Watkins, M. C.; Wysocki, V. H.; Gratton, E.; Nowick, J. S., Elucidating the Oligomerization and Cellular Interactions of a Trimer Derived from Aβ through Fluorescence and Mass Spectrometric Studies. ACS Chem. Neurosci. 2022, 13, 16, 2473–2482 DOI:10.1021/acschemneuro.2c00313. [Request reprint © (pdf)]
Seffernick, J. T.; Turzo, S. M. B. A.; Harvey, S. R.; Kim, Y.; Somogyi, Á.; Marciano, S.; Wysocki, V. H.; Lindert, S., Simulation of Energy-Resolved Mass Spectrometry Distributions from Surface-Induced Dissociation. Analytical Chemistry 2022, 94 (29), 10506-10514. DOI:10.1021/acs.analchem.2c01869. [Request reprint © (pdf)]
Phan, H.-D.; Norris, A. S.; Du, C.; Stachowski, K.; Khairunisa, Bela H.; Sidharthan, V.; Mukhopadhyay, B.; Foster, Mark P.; Wysocki, Vicki H.; Gopalan, V., Elucidation of structure–function relationships in Methanocaldococcus jannaschii RNase P, a multi-subunit catalytic ribonucleoprotein. Nucleic Acids Research 2022, 50 (14), 8154-8167. DOI:10.1093/nar/gkac595. [Request reprint © (pdf)]
Harvey, S. R.; Ben-Nissan, G.; Sharon, M.; Wysocki, V. H., Surface-Induced Dissociation for Protein ComplexProtein complexes Characterization. In Proteoform Identification: Methods and Protocols, Sun, L.; Liu, X., Eds. Springer US: New York, NY, 2022; pp 211-237. DOI:10.1007/978-1-0716-2325-1. [Request reprint © (pdf)]
Geiser, D. L.; Li, W.; Pham, D. Q. D.; Wysocki, V. H.; Winzerling, J. J., Shotgun and TMT-Labeled Proteomic Analysis of the Ovarian Proteins of an Insect Vector, Aedes aegypti (Diptera: Culicidae). Journal of Insect Science 2022, 22 (2), 7. DOI:10.1093/jisesa/ieac018. [Request reprint © (pdf)]
Szkoda, B. E.; Di Capua, A.; Shaffer, J.; Behrman, E. J.; Wysocki, V. H.; Gopalan, V., Characterization of a Salmonella Transcription Factor-DNA Complex and Identification of the Inducer by Native Mass Spectrometry. Journal of Molecular Biology 2022, 434 (7), 167480. DOI:10.1016/j.jmb.2022.167480. [Request reprint © (pdf)]
Stachowski K, Norris AS, Potter D, Wysocki VH, Foster MP. Mechanisms of Cre recombinase synaptic complex assembly and activation illuminated by Cryo-EM. Nucleic Acids Res. 2022 Feb 22;50(3):1753-1769. doi: 10.1093/nar/gkac032. [Request reprint © (pdf)]
Harvey, S. R.; O’Neale, C.; Schey, K. L.; Wysocki, V. H., Native Mass Spectrometry and Surface Induced Dissociation Provide Insight into the Post-Translational Modifications of Tetrameric AQP0 Isolated from Bovine Eye Lens. Analytical Chemistry 2022, 94 (3), 1515-1519. DOI:10.1021/acs.analchem.1c04322. [Request reprint © (pdf)]
Baniasad, M.; Kim, Y.; Shaffer, M.; Sabag-Daigle, A.; Leleiwi, I.; Daly, R. A.; Ahmer, B. M. M.; Wrighton, K. C.; Wysocki, V. H., Optimization of proteomics sample preparation for identification of host and bacterial proteins in mouse feces. Analytical and Bioanalytical Chemistry 2022. 1-15. DOI:10.1007/s00216-022-03885-z. [Request reprint © (pdf)]
Zhang, S.; Yoo, S.; Snyder, D. T.; Katz, B. B.; Henrickson, A.; Demeler, B.; Wysocki, V. H.; Kreutzer, A. G.; Nowick, J. S., A Disulfide-Stabilized Aβ that Forms Dimers but Does Not Form Fibrils. Biochemistry 2022. DOI:10.1021/acs.biochem.1c00739. [Request reprint © (pdf)]
Karch, K. R.; Snyder, D. T.; Harvey, S. R.; Wysocki, V. H., Native Mass Spectrometry: Recent Progress and Remaining Challenges. Annual Review of Biophysics 2022 51:1, 157-179. DOI:10.1146/annurev-biophys-092721-085421. [Request reprint © (pdf)]
Zahurancik, W. J.; Norris, A. S.; Lai, S. M.; Snyder, D. T.; Wysocki, V. H.; Gopalan, V., Chapter Four – Purification, reconstitution, and mass analysis of archaeal RNase P, a multisubunit ribonucleoprotein enzyme. Methods in Enzymology 2021; Vol. 659, pp 71-103. DOI:10.1016/bs.mie.2021.07.006. [Request reprint © (pdf)]
Lai, S. M.; Thirugnanasambantham, P.; Sidharthan, V.; Norris, A. S.; Law, J. D.; Gopalan, V.; Wysocki, V. H., Chapter Three – Use of tandem affinity–buffer exchange chromatography online with native mass spectrometry for optimizing overexpression and purification of recombinant proteins. Methods in Enzymology 2021; Vol. 659, pp 37-70. DOI: 10.1016/bs.mie.2021.07.007. [Request reprint © (pdf)]
Snyder, D. T.; Harvey, S. R.; Wysocki, V. H., Surface-induced Dissociation Mass Spectrometry as a Structural Biology Tool. Chem Rev. 2022 Apr 27;122(8):7442-7487. DOI:10.1021/acs.chemrev.1c00309. [Request reprint © (pdf)]
McCabe, J. W.; Jones, B. J.; Walker, T. E.; Schrader, R. L.; Huntley, A. P.; Lyu, J.; Hoffman, N. M.; Anderson, G. A.; Reilly, P. T. A.; Laganowsky, A.; Wysocki, V. H.; Russell, D. H., Implementing Digital-Waveform Technology for Extended m/z Range Operation on a Native Dual-Quadrupole FT-IM-Orbitrap Mass Spectrometer. Journal of the American Society for Mass Spectrometry 2021, 32 (12), 2812-2820. DOI:10.1021/jasms.1c00245. [Request reprint © (pdf)]
Snyder, D. T.; Jones, B. J.; Lin, Y.-F.; Cooper-Shepherd, D. A.; Hewitt, D.; Wildgoose, J.; Brown, J. M.; Langridge, J. I.; Wysocki, V. H., Surface-induced dissociation of protein complexes on a cyclic ion mobility spectrometer. Analyst 2021,146, 6861-6873 DOI:10.1039/d1an01407b. [Request reprint © (pdf)]
Novikova, I. V.; Zhou, M.; Du, C.; Parra, M.; Kim, D. N.; VanAernum, Z. L.; Shaw, J. B.; Hellmann, H.; Wysocki, V. H.; Evans, J. E., Tunable Heteroassembly of a Plant Pseudoenzyme–Enzyme Complex. ACS Chem. Biol. 2021, 16, 11, 2315–2325 DOI:10.1021/acschembio.1c00475. [Request reprint © (pdf)][Cover article]
Woodall, N. B.; Weinberg, Z.; Park, J.; Busch, F.; Johnson, R. S.; Feldbauer, M. J.; Murphy, M.; Ahlrichs, M.; Yousif, I.; MacCoss, M. J.; Wysocki, V. H.; El-Samad, H.; Baker, D., De novo design of tyrosine and serine kinase-driven protein switches. Nature Structural & Molecular Biology. 2021, 28 (9), 762-770. DOI:10.1038/s41594-021-00649-8. [Request reprint © (pdf)]
Marathe, I. A.; Lai, S. M.; Zahurancik, W. J.; Poirier, M. G.; Wysocki, Vicki H.; Gopalan, V., Protein cofactors and substrate influence Mg2+-dependent structural changes in the catalytic RNA of archaeal RNase P. Nucleic Acids Research. 2021. gkab655. DOI:10.1093/nar/gkab655. [Request reprint © (pdf)]
Baniasad, M.; Reed, A. J.; Lai, S. M.; Zhang, L.; Schulte, K. Q.; Smith, A. R.; LeSassier, D. S.; Weber, K. L.; Hewitt, F. C.; Woerner, A. E.; Gardner, M. W.; Wysocki, V. H.; Freitas, M. A., Optimization of proteomics sample preparation for forensic analysis of skin samples. Journal of Proteomics. 2021, 249, 104360. DOI:10.1016/j.jprot.2021.104360. [Request reprint © (pdf)]
Busch, F.; VanAernum, Z. L.; Lai, S. M.; Gopalan, V.; Wysocki, V. H., Analysis of Tagged Proteins Using Tandem Affinity-Buffer Exchange Chromatography Online with Native Mass Spectrometry. Biochemistry. 2021, 60 (24), 1876-1884. DOI:10.1021/acs.biochem.1c00138. [Request reprint © (pdf)]
Vulovic, I.; Yao, Q.; Park, Y.-J.; Courbet, A.; Norris, A.; Busch, F.; Sahasrabuddhe, A.; Merten, H.; Sahtoe, D. D.; Ueda, G.; Fallas, J. A.; Weaver, S. J.; Hsia, Y.; Langan, R. A.; Plückthun, A.; Wysocki, V. H.; Veesler, D.; Jensen, G. J.; Baker, D., Generation of ordered protein assemblies using rigid three-body fusion. Proceedings of the National Academy of Sciences. 2021, 118 (23), e2015037118. DOI:10.1073/pnas.2015037118. [Request reprint © (pdf)]
Koenig, M. J.; Agana, B. A.; Kaufman, J. M.; Sharpnack, M. F.; Wang, W. Z.; Weigel, C.; Navarro, F. C. P.; Amann, J. M.; Cacciato, N.; Rao Arasada, R.; Gerstein, M. B.; Wysocki, V. H.; Oakes, C.; Carbone, D. P.; STK11/LKB1 loss of function is associated with global DNA hypomethylation and S-adenosyl-methionine depletion in human lung adenocarcinoma. Cancer Res. 2021. DOI: 10.1158/0008-5472.CAN-20-3199. [Request reprint © (pdf)]
Seffernick, J. T.; Canfield, S. M.; Harvey, S. R.; Wysocki, V. H.; Lindert, S.; Prediction-of-protein-complex-structure-using-surface-induced dissociation-and-cryo-electron-microscopy. Anal. Chem. 2021, 93, 7596-7605. DOI: 10.1021/acs.analchem.0c05468. [Request reprint © (pdf)]
Harvey, S. R.; VanAernum, Z. L.; Wysocki, V. H.; Surface-induced dissociation of anionic vs cationic native-like protein complexes. J. Am. Chem. Soc. 2021, 143, 7698-7706. DOI: 10.1021/jacs.1c00855. [Request reprint © (pdf)]
Sahtoe, D. D; Coscia, A.; Mustafaoglu, N.; Miller, L. M.; Olal, D.; Vulovic, I.; Yu, T.-Y.; Goreshnik, I.; Lin, Y.-R.; Clark, L.; Busch, F.; Stewart, L.; Wysocki, V. H.; Ingber, D. E.; Abraham, J.; Baker, D.; Transferring receptor targeting by de novo sheet extension. Proc. Natl. Acad. Sci. USA 2021, 118, e2021569118. DOI: 10.1073/pnas.2021569118. [Request reprint © (pdf)]
McCabe, J. W.; Shirzadeh, M.; Walker, T. E.; Lin, C.-W.; Jones, B. J.; Wysocki, V. H.; Barondeau, D. P.; Clemmer, D. E.; Laganowsky, A.; Russell, D. H.; Variable-temperature electrospray ionization for temperature-dependent folding/refolding reactions of proteins and ligand binding. Anal. Chem. 2021, 93, 6924-6931. DOI: 10.1021/acs.analchem.1c00870. [Request reprint © (pdf)]
Landeras-Bueno, S.; Wasserman, H.; Oliveira, G.; VanAernum, Z. L.; Busch, F.; Salie, Z. L.; Wysocki, V. H.; Andersen, K.; Ollmann Saphire, E.; Cellular mRNA triggers structural transformation of Ebola virus matrix protein VP40 to its essential regulatory form. Cell Rep. 2021, 35, 108986. DOI: 10.1016/j.celrep.2021.108986. [Request reprint © (pdf)]
Caldwell, B. J.; Norris, A.; Zakharova, E.; Smith, C. E.; Wheat, C. T.; Choudhary, D.; Sotomayor, M.; Wysocki, V. H.; Bell, C. E.; Oligomeric complexes formed by Redβ single strand annealing protein in its different DNA bound states. Nucleic Acids Res. 2021, 49(6), 3441-3460. DOI: 10.1093/nar/gkab125. [Request reprint © (pdf)]
Panczyk, E. M.; Snyder, D. T.; Ridgeway, M. E.; Somogyi, A.; Park, M. A.; Wysocki, V. H.; Surface-induced dissociation of protein complexes selected by trapped ion mobility spectrometry. Anal. Chem. 2021, 93, 5513-5520. DOI: 10.1021/acs.analchem.0c05373. [Request reprint © (pdf)]
Smith, H.; Pinkerton, N.; Heisler, D. B.; Kudryashova, E.; Hall, A. R.; Karch, K. R.; Norris, A.; Wysocki, V.; Sotomayor, M.; Reisler, E.; Vavylonis, D.; Kudryashov, D. S.; Rounding out the understanding of ACD toxicity with the discovery of cyclic forms of actin oligomers. Int. J. Mol. Sci. 2021, 22, 718. DOI: 10.3390/ijms22020718. [Request reprint © (pdf)]
Vorobieva, A. A.; White, P.; Liang, B.; Horne, J. E.; Bera, A. K.; Chow, C. M.; Gerben, S.; Marx, S.; Kang, A.; Stiving, A. Q.; Harvey, S. R.; Marx, D. C.; Khan, G. N.; Fleming, K. G.; Wysocki, V. H.; Brockwell, D. J.; Tamm, L. K.; Radford, S. E.; Baker, D. De Novo Design of Transmembrane β-Barrels, Science, 2021, 371 (6531), eabc8182. DOI: 10.1126/science.abc8182. [Request reprint © (pdf)]
Snyder, D. T.; Harvey, S. R.; Busch, F.; Wysocki, V. H. Chapter 11: Surface-Induced Dissociation in Biomolecular Mass Spectrometry. In Advanced Fragmentation Methods. Biomolecular Mass Spectrometry; 2020; pp 281–336. DOI: 10.1039/9781839161056-00281.
Snyder, D. T.; Lin, Y.-F.; Somogyi, A.; Wysocki, V. H.; Tandem surface-induced dissociation of protein complexes on an ultrahigh resolution platform. International Journal of Mass Spectrometry. 2021, 461, 116503. DOI: 10.1016/j.ijms.2020.116503. [Request reprint © (pdf)] [Cover article]
Harvey, S.R.; VanAernum, Z.L.; Kostelic, M.M.; Marty, M.T. and Wysocki, V.H.; Probing the structure of nanodiscs using surface-induced dissociation mass spectrometry. Chemical Communications. 2020, 56(100), 15651-15654. DOI: 10.1039/D0CC05531J. [Request reprint © (pdf)]
Agana, B.A.; Wysocki, V.H. and Schoenberg, D.R.; Cytoplasmic mRNA recapping has limited impact on proteome complexity. Open Biology. 2020, 10(11), 200313. DOI: 0.1098/rsob.200313. [Request reprint © (pdf)]
Stiving, A.Q.; Harvey, S.R.; Jones, B.J.; Bellina, B.; Brown, J.M.; Barran, P.E. and Wysocki, V.H.; Coupling 193 nm Ultraviolet Photodissociation and Ion Mobility for Sequence Characterization of Conformationally-Selected Peptides. J. Am. Soc. Mass Spectrom. 2020, 31(11),2313-2320. DOI: 10.1021/jasms.0c00259.[Request reprint © (pdf)]
Sarni, S.; Biswas, B.; Liu, S.; Olson, E. D.; Kitzrow, J.; Rein, A.; Wysocki, V.; Musier-Forsyth, K.; HIV-1 Gag protein with or without p6 specifically dimerizes on the packaging signal in viral RNA. J. Biol. Chem. 2020, 295(42),14391-14401. DOI: 10.1074/jbc.RA120.014835. [Request reprint © (pdf)]
Snyder, D. T.; Panczyk, E. M.; Somogyi, A.; Kaplan, D. A.; Wysocki, V.; Simple and Minimally Invasive SID Devices for Native Mass Spectrometry. Analytical Chemistry 2020,92, 16, 11195–11203. DOI: 10.1021/acs.analchem.0c01657. [Request reprint © (pdf)]
Holmquist, M.; Ihms, E. C., Gollnick, P.; Wysocki, V. H.; and Foster, M. P.; Population distributions from native mass spectrometry titrations reveal nearest-neighbor cooperativity in the ring-shaped oligomeric protein TRAP. Biochemistry. 2020, 59, 27, 2518–252. DOI: 10.1021/acs.biochem.0c00352. [Request reprint © (pdf)]
Bansal, P.; Yatsyna, V.; AbiKhodr, A. H.; Warnke, S.; Ben Faleh, A.; Yalovenko, N.; Wysocki, V. H. and Rizzo, T. R.; Using SLIM-based IMS-IMS together with cryogenic infrared spectroscopy for glycan analysis. Analytical Chemistry. 2020, 92, 9079−9085. DOI: 10.1021/acs.analchem.0c01265. [Request reprint © (pdf)]
Vimer, S.; Ben-Nissan, G.; Morgenstern, D.; Kumar-Deshmukh, F.; Polkinghorn, C.; Quintyn, R. S.; Vasil’ev, Y. V.; Beckman, J. S.; Elad, N.; Wysocki, V. H.; and Sharon, M.; Comparative structural analysis of 20S proteasome ortholog protein complexes by native mass spectrometry. ACS Central Science. 2020, 6(4): 573-588. DOI: 10.1021/acscentsci.0c00080. [Request reprint © (pdf)]
Chen, Z.; Kibler, R.; Hunt, A.; Busch, F.; Pearl, J.; Jia, M.; VanAernum, Z. L.; Wicky, B. I. M.; Dods, G.; Liao, H.; Wilken, M. S.; Ciarlo, C.; Green, S.; El-Samad, H.; Stamatoyannopoulos, J.; Wysocki, V. H.; Jewett, M. C.; Boyken, S. E.; and Baker, D.; De novo design of protein logic gates. Science. 2020, 368 (6486): 78-84. DOI: 10.1126/science.aay2790. [Request reprint © (pdf)]
Jia, M.; Sen, S.; Wachnowsky, C.; Fidai, I.; Cowan, J.A. and Wysocki, V. H.; Characterization of [2Fe‐2S]‐Cluster‐Bridged Protein Complexes and Reaction Intermediates by use of Robust Native Mass Spectrometric Methods. Angewandte Chemie International Edition. 2020, 59 (17), 6724-6728. DOI: 10.1002/anie.201915615.[Request reprint © (pdf)]
Stiving, A. Q.; Jones, B. J.; Ujma, J.; Giles, K. and Wysocki, V. H.; Collision Cross Sections of Charge-Reduced Proteins and Protein Complexes: a Database for CCS Calibration. Analytical Chemistry. 2020, 92, 4475-4483. DOI: 10.1021/acs.analchem.9b05519. [Request reprint © (pdf)]
Norris, A.; Busch, F.; Schupfner, M.; Sterner, R. and Wysocki, V. H.; Quaternary Structure of the Tryptophan Synthase α‑Subunit Homolog BX1 from Zea mays. J. Am. Soc. Mass Spectrom. 2020, 31(2):227-233. DOI: 10.1021/jasms.9b00068. [Request reprint © (pdf)]
Panczyk, E.M.; Gilbert, J. D.; Jagdale, G. S.; Stiving, A. Q.; Baker, L. A. and Wysocki, V. H.; Ion Mobility and Surface Collisions: Submicrometer Capillaries Can Produce Native-like Protein Complexes. Anal Chem. 2020, 92(3):2460-2467. DOI: 10.1021/acs.analchem.9b03666. [Request reprint © (pdf)]
VanAernum, Z.; Busch, F.; Jones, B. J.; Jia, M.; Chen, Z.; Boyken, S. E.; Sahasrabuddhe, A.; Baker, D.; Wysocki, V. H. Rapid Online Buffer Exchange: A Method for Screening of Proteins, Protein Complexes, and Cell Lysates by Native Mass Spectrometry. Nat Protoc. 2020, 15, 1132-1157. DOI: 10.1038/s41596-019-0281-0. [Request reprint © (pdf)]
Stiving, A.Q.; Gilbert, J.D.; Jones, B. J.; Wysocki, V. H.; A Tilted Surface and Ion Carpet Array for SID. J. Am. Soc. Mass Spectrom. 2020, 31(2):458-462 DOI: 10.1021/jasms.9b00009. [Request reprint © (pdf)]
Kang, M.; Doddapaneni, K.; Sarni, S.; Heppner, Z.; Wysocki, V.; and Wu, Z.; Solution Structure of the Nucleotide hydrolase BlsM: Implication of its Substrate Specificity. Protein Science. 2019,29 (8), 1760-1763. DOI: 10.1002/pro.3812. [Request reprint © (pdf)]
Cardona, A.; O’Brien, A.; Bernier, M.C.; Somogyi, A.; Wysocki, V.H.; Smark S.; He, X.; Ambrosia, G.; Hsueh, W.A.; Raman, S.V. Trimethylamine N-oxide and incident atherosclerotic events in high-risk individuals with diabetes: an ACCORD trial post hoc analysis. BMJ Open Diab Res Care. 2019; 7 (1): e000718. DOI: 10.1136/bmjdrc-2019-000718 [Request reprint © (pdf)]
Purde, V.; Busch, F.; Kudryashova, E.; Wysocki, V. H. and Kudryashov, D. S.; Oligomerization Affects the Ability of Human Cyclase-Associated Proteins 1 and 2 to Promote Actin Severing by Cofilins. Int J Mol Sci. 2019, 20(22):5647. DOI: 10.3390/ijms20225647 [Request reprint © (pdf)]
Kneuttinger, A. C.; Zwisele, S.; Straub, K.; Bruckmann, A.; Busch, F.; Kinateder, T.; Gaim, B.; Wysocki, V. H.; Merkl, R.; Sterner, R.; Light-Regulation of Tryptophan Synthase by Combining Protein Design and Enzymology. Int J Mol Sci. 2019, 20(20): 5106. DOI: 10.3390/ijms20205106 [Request reprint © (pdf)]
Snyder, D.T.; Panczyk, E.; Stiving, A.Q.; Gilbert, J.D.; Somogyi, A.; Kaplan, D.; Design and Performance of a Second-Generation Surface-Induced Dissociation Cell for Fourier Transform Ion Cyclotron Resonance Mass Spectrometry of Native Protein Complexes. Anal. Chem. 2019, 91(21):14049-14057. DOI: 10.1021/acs.analchem.9b03746 [Request reprint © (pdf)]
Kneuttinger, A.C.; Straub, K.; Bittner, P.; Simeth, N.A.; Bruckmann, A.; Busch, F.; Rajendran, C.; Hupfeld, E.; Wysocki, V.H.; Horinek, D.; König, B., Light Regulation of Enzyme Allostery through Photo-responsive Unnatural Amino Acids. Cell Chem Biol. 2019, 26(11):1501-1514. DOI: 10.1016/j.chembiol.2019.08.006 [Request reprint © (pdf)]
Sengupta, A.; Wu, J.; Seffernick, J.T.; Sabag-Daigle, A.; Thomsen, N.; Chen, T.H.; Di Capua, A.; Bell, C.E.; Ahmer, B.M.; Lindert, S.; Wysocki, V.H.; Gopalan V., Integrated use of biochemical, native mass spectrometry, computational and genome-editing methods to elucidate the mechanism of a Salmonella deglycase. Journal of molecular biology. J Mol Biol. 2019, 431(22):4497-4513. DOI: 10.1016/j.jmb.2019.08.017 [Request reprint © (pdf)]
Seffernick, J. T.; Harvey, S. R.; Wysocki, V. H.; Lindert, S., Predicting Protein Complex Structure from Surface-Induced Dissociation Mass Spectrometry Data. ACS Cent Sci. 2019, 5(8):1330-1341. DOI: 10.1021/acscentsci.8b00912.[Request reprint © (pdf)]
Smith, Z.M; Wang, X.; Scheerer, J. R.; Martens, J.; Berden, G.; Oomens, J.; Steinmetz, V.; Somogyi, A.; Wysocki, V. H.; Poutsma, J. C., Spectroscopic Evidence for Lactam Formation in Terminal Ornithine b2+ and b3+ Fragment Ions. J. Am. Soc. Mass Spectrom. 2019, 30(9):1565-1577. DOI: 10.1007/s13361-019-02244-0. [Request reprint © (pdf)]
Boyken, S. E.; Benhaim, M. A.; Busch, F.; Jia, M.; Bick, M. J.; Choi, H.; Klima, J. C.; Chen, Z.; Walkey, C.; Mileant, A.; Sahasrabuddhe, A.; Wei, K. Y.; Hodge, E. A.; Byron, S.; Quijano-Rubio, A.; Sankaran, B.; King, N. P.; Lippincott-Schwartz, J.; Wysocki, V. H.; Lee, K.K.; Baker D., De novo design of tunable, pH-driven conformational changes. Science. 2019, 364(6441):658-664. DOI: 10.1126/science.aav7897. [Request reprint © (pdf)]
Schupfner, M.; Busch F.; Wysocki, V.; Sterner, R., Generation of a stand-alone tryptophan synthase α-subunit by mimicking an evolutionary blueprint. Chembiochem. 2019, 20(21):2747-2751. DOI: 10.1002/cbic.201900323. [Request reprint © (pdf)]
Harvey, S. R.; Seffernick, J. T.; Quintyn, R. S.; Song, Y.; Ju, Y.; Yan, J.; Sahasrabuddhe, A. N.; Norris, A.; Zhou, M.; Behrman, E. J.; Lindert, S.; Wysocki, V. H., Relative interfacial cleavage energetics of protein complexes revealed by surface collisions. PNAS. 2019, 116(17):8143-8148. DOI: 10.1073/pnas.1817632116. [Request reprint © (pdf)]
VanAernum, Z. L.; Gilbert, J. D.; Belov, M. E.; Makarov, A. A.; Horning, S. R.; Wysocki, V. H., Surface-Induced Dissociation of Noncovalent Protein Complexes in an Extended Mass Range Orbitrap Mass Spectrometer. Anal Chem. 2019, 91(5):3611-3618. DOI: 10.1021/acs.analchem.8b05605. . [Request reprint © (pdf)]
Chen, Z.; Boyken, S. E.; Jia, M.; Busch, F.; Flores-Solis, D.; Bick, M. J.; Lu, P.; VanAernum, Z. L.; Sahasrabuddhe, A.; Langan, R. A.; Bermeo, S.; Brunette, T. J.; Mulligan, V. K.; Carter, L. P.; DiMaio, F.; Sgourakis, N. G.; Wysocki, V. H.; Baker, D., Programmable design of orthogonal protein heterodimers. Nature. 2019, 565(7737):106-111. DOI: 10.1038/s41586-018-0802-y. [Request reprint © (pdf)]
Chen, L.; Tanimoto, A.; So, B. R.; Bakhtina, M.; Magliery, T. J.; Wysocki, V. H.; Musier-Forsyth, K., Stoichiometry of triple-sieve tRNA editing complex ensures fidelity of aminoacyl-tRNA formation. Nucleic Acids Res. 2019,47(2):929-940. DOI: 10.1093/nar/gky1153. [Request reprint © (pdf)]
Stiving, A. Q.; VanAernum Z.; Busch, F.; Harvey S. R.; Sarni, S.; Wysocki, V. H., Surface-Induced Dissociation: An Effective Method for Characterization of Protein Quaternary Structure. Anal Chem. 2019, 91(1):190-209. DOI: 10.1021/acs.analchem.8b05071. [Request reprint © (pdf)]
Mabin, J. W.; Woodward, L. A.; Patton, R. D.; Yi, Z.; Jia, M.; Wysocki, V. H.; Bundschuh, R.; Singh, G., The Exon Junction Complex Undergoes a Compositional Switch that Alters mRNP Structure and Nonsense-Mediated mRNA Decay Activity. Cell Rep. 2018, 25(9):2431-2446.e7. DOI: 10.1016/j.celrep.2018.11.046. [Request reprint © (pdf)]
Busch, F.; VanAernum, Z. L.; Ju, Y.; Yan, J.; Gilbert, J. D.; Quintyn, R. S.; Bern, M.; Wysocki, V. H., Localization of protein complex bound ligands by surface-induced dissociation high-resolution mass spectrometry, Anal Chem. 2018, 90(21):12796-12801. DOI: 10.1021/acs.analchem.8b03263. [Request reprint © (pdf)]
Trotman, J. B.; Agana, B. A.; Giltmier, A. J.; Wysocki, V. H.; Schoenberg, D. R., RNA-binding proteins and heat-shock protein 90 are constituents of the cytoplasmic capping enzyme interactome, JBC. 2018, 293(43):16596-16607. DOI: 10.1074/jbc.RA118.004973. [Request reprint © (pdf)]
Sharpnack, M. F.; Ranbaduge, N.; Srivastava, A.; Cerciello, F.; Codreanu, S. G.; Liebler, D. C.; Mascaux, C.; Miles, W. O.; Morris, R.; McDermott, J. E.; Sharpnack, J. L.; Amann, J.; Maher, C. A.; Machiraju, R.; Wysocki, V. H.; Govindan, R.; Mallick, P.; Coombes, K. R.; Huang, K.; Carbone, D. P., Proteogenomic Analysis of Surgically Resected Lung Adenocarcinoma, J Thorac Oncol. 2018, 13(10):1519-1529. DOI: 10.1016/j.jtho.2018.06.025. [Request reprint © (pdf)]
Gadkari, V. V.; Harvey, S. R.; Raper, A. T.; Chu, W. T.; Wang, J.; Wysocki, V. H.; Suo, Z., Investigation of sliding DNA clamp by single-molecule fluorescence, mass spectrometry and structure-based modeling, Nucleic Acids Res. 2018, 46(6):3103-3118. DOI: 10.1093/nar/gky125. [Request reprint © (pdf)]
Wu, J.; Sabag-Daigle, A.; Borton, M. A.; Kop, L. F. M.; Szkoda, B. E.; Deatherage Kaiser, B. L.; Lindemann, S. R.; Renslow, R. S.; Wei, S.; Nicora, C. D.; Weitz, K. K.; Kim, Y. M.; Adkins, J. N.; Metz, T. O.; Boyaka, P.; Gopalan, V.; Wrighton, K. C.; Wysocki, V. H.; Ahmer, B. M. M., Salmonella-Mediated Inflammation Eliminates Competitors for Fructose-Asparagine in the Gut, Infect Immun. 2018, 86(5). pii: e00945-17. DOI: 10.1128/IAI.00945-17. [Request reprint © (pdf)]
Aebersold, R.; Agar, J. N.; Amster, I. J. A.; Baker, M. S. A.; Bertozzi, C. R.; Boja, E. S.; Costello, C. E.; Cravatt, B. F. A.; Fenselau, C.; Garcia, B. A.; Ge, Y.; Gunawardena, J.; Hendrickson, R. C.; Hergenrother, P. J.; Huber, C. G. A.; Ivanov, A. R.; Jensen, O. N.; Jewett, M. C.; Kelleher, N. L. A.; Kiessling, L. L. A.; Krogan, N. J.; Larsen, M. R.; Loo, J. A. A.; Ogorzalek Loo, R. R.; Lundberg, E.; MacCoss, M. J.; Mallick, P.; Mootha, V. K.; Mrksich, M.; Muir, T. W.; Patrie, S. M.; Pesavento, J. J. A.; Pitteri, S. J. A. X.; Rodriguez, H.; Saghatelian, A.; Sandoval, W.; Schluter, H. A.; Sechi, S.; Slavoff, S. A.; Smith, L. M.; Snyder, M. P.; Thomas, P. M. A.; Uhlen, M.; Van Eyk, J. E.; Vidal, M.; Walt, D. R.; White, F. M.; Williams, E. R.; Wohlschlager, T.; Wysocki, V. H.; Yates, N. A.; Young, N. L. A.; Zhang, B. A., How Many Human Proteoforms Are There? Nat Chem Biol. 2018, 14(3):206-214. DOI: 10.1038/nchembio.2576. [Request reprint © (pdf)]
Zhou, M.; Yan, J.; Romano, C. A.; Tebo, B. M.; Wysocki, V. H.;
Sahasrabuddhe, A.; Hsia, Y.; Busch, F.; Sheffler, W.; King, N. P.; Baker, D.; Wysocki, V. H., Confirmation of intersubunit connectivity and topology of designed protein complexes by native MS, PNAS. 2018, 115(6):1268-1273. DOI: 10.1073/pnas.1713646115. [Request reprint © (pdf)]
Wu, J.; Sabag-Daigle, A.; Metz, T. O.; Deatherage Kaiser, B. L.; Gopalan, V.; Behrman, E. J.; Wysocki, V. H.; Ahmer, B. M. M., Measurement of Fructose-Asparagine Concentrations in Human and Animal Foods, J. Agric. Food Chem. 2018, 66(1):212-217. DOI: 10.1021/acs.jafc.7b04237.[Request reprint © (pdf)]
Sabag-Daigle, A.; Wu, J.; Borton, M. A.; Sengupta, A.; Gopalan, V.; Wrighton, K. C.; Wysocki, V. H.; Ahmer, B. M. M., Identification of bacterial species that can utilize fructose-asparagine, Appl Environ Microbiol. 2017, 84(5). pii: e01957-17. DOI: 10.1128/AEM.01957-17.[Request reprint © (pdf)]
Human Argonaute3 has slicer activity, Nucleic Acids Research 2017, 45(20):11867-11877. DOI: 10.1093/nar/gkx916Bungard, D.; Copple, J. S.; Yan, J.; Chhun, J. J.; Kumirov, V. K.; Foy, S. G.; Masel, J.; Wysocki, V. H.; Cordes, M. H. J., Foldability of a Natural De Novo Evolved Protein, Structure 2017, 25(11):1687-1696.e4. DOI: 10.1016/j.str.2017.09.006. [Request reprint © (pdf)]
Romano, C. A.; Zhou, M.; Song, Y.; Wysocki, V. H.; Dohnalkova, A. C.; Kovarik, L.; Paša-Tolić, L.; Tebo, B. M., Biogenic manganese oxide nanoparticle formation by a multimeric multicopper oxidase Mnx, Nature Communications , 2017, 8(1):746. DOI: 10.1038/s41467-017-00896-8, [Request reprint © (pdf)]
Plach, M. G.; Semmelmann, F.; Busch, F.; Busch, M.; Heizinger, L.; Wysock, V. H.; Merkl, R.; Sterner, R., Evolutionary diversification of protein-protein interactions by interface add-ons, Proc Nat Acad Sci, 2017, 114(40):E8333-E8342. DOI: 10.1073/pnas.1707335114. [Request reprint © (pdf)]
Harvey, S. R.; Liu, Y.; Liu, W.; Wysocki, V. H., Laganowsky, A.; Surface induced dissociation as a tool to study membrane protein complexes, ChemComm., 2017, 53(21):3106-3109. DOI: 10.1039/C6CC09606A. [Request reprint © (pdf)]